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Literature summary for 1.14.14.145 extracted from

  • Funk, C.; Croteau, R.
    Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis (1994), Arch. Biochem. Biophys., 308, 258-266.
    View publication on PubMed
Search for more literature for 1.14.14.145

Inhibitors

Inhibitors Comment Organism Structure
Ancymidol
-
 Abies grandis
clotrimazole
-
 Abies grandis
CO blue light reversible inhibition Abies grandis
cytochrome c
-
 Abies grandis
Metyrapone
-
 Abies grandis
miconazole
-
 Abies grandis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 abietadienol pH 7.5, 32°C Abies grandis

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Abies grandis
-
-
microsome
-
 Pinus contorta
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 Pinus contorta this enzyme catalyzes a step in the pathway of abietic acid biosynthesis abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 Abies grandis this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Abies grandis
-
 Lindl.
-
Pinus contorta
-
 Douglas var. latifolia Engelman
-

Source Tissue

Source Tissue Comment Organism Textmining
stem cell-free stem extracts Abies grandis
-
stem cell-free stem extracts Pinus contorta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 this enzyme catalyzes a step in the pathway of abietic acid biosynthesis Pinus contorta abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
abieta-7,13-dien-18-ol + [reduced NADPH-hemoprotein reductase] + O2 this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant Abies grandis abieta-7,13-dien-18-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
abieta-7,13-dien-18-ol hydroxylase
-
 Abies grandis
abieta-7,13-dien-18-ol hydroxylase
-
 Pinus contorta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
32
-
 assay at Abies grandis
32
-
 assay at Pinus contorta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.6
-
 Abies grandis
7.6
-
 assay at Pinus contorta

pH Range

pH Minimum pH Maximum Comment Organism
7 8 60-70% of maximal activity at pH 7.0 and 8.0 Abies grandis

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 cytochrome P-450-dependent monooxygenase Abies grandis
FAD omission of flavins results in measurable reduction in both hydroxylation activities Abies grandis
FMN omission of flavins results in measurable reduction in both hydroxylation activities Abies grandis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 no inhibition detected by ancymidol at 0.2 mM Abies grandis additional information
0.0013
-
 pH 7.5, 32°C Abies grandis clotrimazole
0.004
-
 pH 7.5, 32°C Abies grandis cytochrome c
0.005
-
 pH 7.5, 32°C Abies grandis Metyrapone
0.307
-
 pH 7.5, 32°C Abies grandis miconazole