Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ancymidol | - |
Abies grandis | |
clotrimazole | - |
Abies grandis | |
CO | blue light reversible inhibition | Abies grandis | |
cytochrome c | - |
Abies grandis | |
Metyrapone | - |
Abies grandis | |
miconazole | - |
Abies grandis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
abietadiene | pH 7.5, 32°C | Abies grandis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Abies grandis | - |
- |
microsome | - |
Pinus contorta | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | Pinus contorta | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | Abies grandis | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | - |
Lindl. | - |
Pinus contorta | - |
Douglas var. latifolia Engelman | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | cell-free stem extracts | Abies grandis | - |
stem | cell-free stem extracts | Pinus contorta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pinus contorta | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis | Pinus contorta | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
abietadiene + [reduced NADPH-hemoprotein reductase] + O2 | this enzyme catalyzes a step in the pathway of abietic acid biosynthesis. Hydroxylation activity is maximally supported by NADPH, and NADH is less effective as reductant | Abies grandis | abietadienol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
abietadiene-18-hydroxylase | - |
Abies grandis |
abietadiene-18-hydroxylase | - |
Pinus contorta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
assay at | Abies grandis |
32 | - |
assay at | Pinus contorta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.6 | - |
Abies grandis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 60-70% of maximal activity at pH 7.0 and 8.0 | Abies grandis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | cytochrome P-450-dependent monooxygenase | Abies grandis | |
FAD | omission of flavins results in measurable reduction in both hydroxylation activities | Abies grandis | |
FMN | omission of flavins results in measurable reduction in both hydroxylation activities | Abies grandis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0005 | - |
pH 7.5, 32°C | Abies grandis | clotrimazole | |
0.0009 | - |
pH 7.5, 32°C | Abies grandis | cytochrome c | |
0.005 | - |
pH 7.5, 32°C | Abies grandis | Metyrapone | |
0.0093 | - |
pH 7.5, 32°C | Abies grandis | Ancymidol | |
0.307 | - |
pH 7.5, 32°C | Abies grandis | miconazole |