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Literature summary for 1.14.14.143 extracted from

  • Bertea, C.M.; Schalk, M.; Karp, F.; Maffei, M.; Croteau, R.
    Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene (2001), Arch. Biochem. Biophys., 390, 279-286.
    View publication on PubMed
Search for more literature for 1.14.14.143

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determinationand analysis, functional expression in Saccharomyces cerevisiae strains WAT11U and WAT21U, which constitutively express two different isoforms of the Arabidposis thaliana NADPH-cytochrome P450 reductase, and in Escherichia coli strain JM109 Mentha pulegium

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Mentha x piperita
-
-
microsome
-
 Mentha pulegium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Mentha x piperita
Fe2+
-
 Mentha pulegium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55360
-
 x * 55360, sequence calculation Mentha pulegium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 Mentha x piperita
-
 (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 Mentha pulegium
-
 (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mentha pulegium
-
-
-
Mentha x piperita
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Mentha x piperita
-
leaf
-
 Mentha pulegium
-
plant oil gland secretory cells Mentha x piperita
-
plant oil gland secretory cells Mentha pulegium
-
secretory cell
-
 Mentha x piperita
-
secretory cell
-
 Mentha pulegium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
 Mentha x piperita (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
 Mentha pulegium (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan Mentha x piperita (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan Mentha pulegium (+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O GC-MS product analysis ?

Subunits

Subunits Comment Organism
? x * 55360, sequence calculation Mentha pulegium

Synonyms

Synonyms Comment Organism
(+)-pulegone-9-hydroxylase
-
 Mentha x piperita
(+)-pulegone-9-hydroxylase
-
 Mentha pulegium
menthofuran synthase
-
 Mentha x piperita
menthofuran synthase
-
 Mentha pulegium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mentha pulegium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mentha pulegium

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 a cytochrome P450 monooxygenase Mentha x piperita
cytochrome P450 a cytochrome P450 monooxygenase Mentha pulegium
FAD
-
 Mentha pulegium
FMN
-
 Mentha pulegium