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Literature summary for 1.14.14.141 extracted from

  • Hamerski, D.; Matern, U.
    Elicitor-induced biosynthesis of psoralens in Ammi majus L. suspension cultures. Microsomal conversion of demethylsuberosin into (+)marmesin and psoralen (1988), Eur. J. Biochem., 171, 369-375.
    View publication on PubMed
Search for more literature for 1.14.14.141

Activating Compound

Activating Compound Comment Organism Structure
additional information elicitor preparations from either Alternaria carthami Chowdhury or Phytophthora megasperma f.sp. glycinea induce the production of linear furanocoumarins in Ammi majus suspension culture Ammi majus

Inhibitors

Inhibitors Comment Organism Structure
Ancymidole 13% inhibition of the psoralen synthase at 0.05 mM Ammi majus
BAS 110 72% inhibition of the psoralen synthase at 0.05 mM, 38% at 0.005 mM Ammi majus
BAS 111 complete inhibition of the psoralen synthase at 0.001 mM, 86% at 0.0002 mM Ammi majus
BAS 978 complete inhibition of the psoralen synthase at 0.05 mM, 68% at 0.005 mM Ammi majus
ketoconazole complete inhibition of the psoralen synthase at 0.001 mM, 74% at 0.0002 mM Ammi majus
Tetcyclacis 85% inhibition of the psoralen synthase at 0.001 mM, 25% at 0.0002 mM Ammi majus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum lumen
-
 Ammi majus 5788
-
microsome
-
 Ammi majus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 Ammi majus the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
 ?
additional information Ammi majus umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ammi majus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by preparation of microsomes Ammi majus

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Ammi majus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
 Ammi majus psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
 ?
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium Ammi majus psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
 ?
additional information umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin Ammi majus ?
-
 ?
additional information the psoralen synthase is a cytochrome-P450-dependent monooxygenase Ammi majus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Ammi majus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Ammi majus

Cofactor

Cofactor Comment Organism Structure
additional information the psoralen synthase is a cytochrome-P450-dependent monooxygenase Ammi majus