Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.141 extracted from

  • Wendorff, H.; Matern, U.
    Differential response of cultured parsley cells to elicitors from two non-pathogenic strains of fungi. Microsomal conversion of (+)marmesin into psoralen (1986), Eur. J. Biochem., 161, 391-398.
    View publication on PubMed
Search for more literature for 1.14.14.141

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect on enzyme activity by 2-mercaptoethanol and DTT, elicitor fractions isolated from cell walls of Alternaria carthami Chowdhury and of Phythophthora megasperma Drechsler f. sp. glycinea induce the enzyme, the endoplasmic reticulum of cultured parsley cells is the primary target in the differential induction by elicitors from these two non-pathogenic strains of fungi. Petroselinum crispum
NADH NADH acts synergistically with NADPH, but cannot substitute for NADPH Petroselinum crispum

Inhibitors

Inhibitors Comment Organism Structure
Ancymidole 48% inhibition of the psoralen synthase at 0.05 mM, 29% at 0.005 mM Petroselinum crispum
BAS 110 34% inhibition of the psoralen synthase at 0.001 mM, 30% at 0.0002 mM Petroselinum crispum
BAS 111 52% inhibition of the psoralen synthase at 0.001 mM, 33% at 0.0002 mM Petroselinum crispum
BAS 978 10% inhibition of the psoralen synthase at 0.001 mM Petroselinum crispum
carbon monoxide
-
 Petroselinum crispum
cytochrome c 72% inhibition at 0.5 nM Petroselinum crispum
ketoconazole 38% inhibition of the psoralen synthase at 0.050 mM, 20% at 0.005 mM Petroselinum crispum
additional information no effect on enzyme activity by 2-mercaptoethanol and DTT Petroselinum crispum
NADP+ 43% product inhibition at 1 mM Petroselinum crispum
Tetcyclacis 47% inhibition of the psoralen synthase at 0.05 mM, 51% at 0.005 mM Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 NADPH pH 7.0, 20°C, microsomes Petroselinum crispum

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Petroselinum crispum 5783
-
microsome
-
 Petroselinum crispum
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ does not affect the enzyme activity Petroselinum crispum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 Petroselinum crispum
-
 psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially by preparation of microsomes Petroselinum crispum

Reaction

Reaction Comment Organism Reaction ID
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 = psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O reaction mechanism Petroselinum crispum
a

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Petroselinum crispum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-marmesin + NADP + H+ + O2
-
 Petroselinum crispum psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + H2O
-
 r
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
 Petroselinum crispum psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
 ?
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 very poor activity with (-)-marmesin, i.e. nodakenetin Petroselinum crispum psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
 ?
additional information the psoralen synthase is a cytochrome-P450-dependent monooxygenase Petroselinum crispum ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Petroselinum crispum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
 short exposure leads to complete inactivation Petroselinum crispum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.25
-
 Petroselinum crispum

Cofactor

Cofactor Comment Organism Structure
additional information the psoralen synthase is a cytochrome-P450-dependent monooxygenase Petroselinum crispum