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Literature summary for 1.14.14.137 extracted from

  • Saito, S.; Hirai, N.; Matsumoto, C.; Ohigashi, H.; Ohta, D.; Sakata, K.; Mizutani, M.
    Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid (2004), Plant Physiol., 134, 1439-1449.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.137

Cloned(Commentary)

Cloned (Comment) Organism
expression in baculovirus system, isoforms CYP707A1, CYP707A3, CYP707A4 Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
cytochrome c 0.1 mM, complete inhibition Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 (+)-S-abscisate pH 7.25, 30°C, isoform CYP707A3 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Arabidopsis thaliana
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 53000, isoform CYC707A1, x * 52000, isoform CYP707A3, SDS-PAGE Arabidopsis thaliana
53000
-
 x * 53000, isoform CYC707A1, x * 52000, isoform CYP707A3, SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 expression in baculovirus system, isoforms CYP707A1, CYP707A3, CYP707A4
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 isoform CYP707A3 is specific for (+)-isomer Arabidopsis thaliana 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O isoform CYP707A3, no hydroxylation at 7’ position ?
additional information isomerisation of 8’-hydroxy-abscisic acid to phaseic acid is not catalyzed by enzyme Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, isoform CYC707A1, x * 52000, isoform CYP707A3, SDS-PAGE Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
additional information NADH may not substitute for NADPH Arabidopsis thaliana