Literature summary for 1.14.14.132 extracted from

Lau, W.; Sattely, E.
Six enzymes from mayapple that complete the biosynthetic pathway to the etoposide aglycone (2015), Science, 349, 1224-1228.

Search for more literature for 1.14.14.132

Application

Application	Comment	Organism
synthesis	recombinant coexpression of the genes involved in the podophyllotoxin pathway permits to reconstitute the pathway to (-)-4'-demethylepipodophyllotoxin (the etoposide aglycone), a naturally occurring lignan that is the immediate precursor of etoposide and, unlike podophyllotoxin, a potent topoisomerase inhibitor, for production of the etoposide aglycone in tobacco and circumvent the need for cultivation of mayapple and semisynthetic epimerization and demethylation of podophyllotoxin	Sinopodophyllum hexandrum

Cloned(Commentary)

Cloned (Comment)	Organism
gene CYP82D61, expression analysis by qRT-PCR analysis, transcriptome mining to identify biosynthetic genes in the podophyllotoxin pathway, Agrobacterium-mediated functional transient expression in Nicotiana benthamiana	Sinopodophyllum hexandrum

Organism

Organism	UniProt	Comment	Textmining
Sinopodophyllum hexandrum	A0A0N7F297	or Sinopodophyllum hexandrum, gene CYP82D61	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(-)-4'-demethyl-deoxypodophyllotoxin + [reduced NADPH-hemoprotein reductase] + O2	-	Sinopodophyllum hexandrum	(-)-4'-demethylepipodophyllotoxin + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

General Information

General Information	Comment	Organism
metabolism	the enzyme is involved in the biosynthesis of (-)-4'-demethylepipodophyllotoxin (the etoposide aglycone), a naturally occurring lignan that is the immediate precursor of etoposide and, unlike podophyllotoxin, a potent topoisomerase inhibitor. Biosynthetic pathway of (-)-podophyllotoxin, overview	Sinopodophyllum hexandrum

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Release 2023.1 (January 2023)