Application | Comment | Organism |
---|---|---|
synthesis | recombinant coexpression of the genes involved in the podophyllotoxin pathway permits to reconstitute the pathway to (-)-4'-demethylepipodophyllotoxin (the etoposide aglycone), a naturally occurring lignan that is the immediate precursor of etoposide and, unlike podophyllotoxin, a potent topoisomerase inhibitor, for production of the etoposide aglycone in tobacco and circumvent the need for cultivation of mayapple and semisynthetic epimerization and demethylation of podophyllotoxin | Sinopodophyllum hexandrum |
Cloned (Comment) | Organism |
---|---|
gene Phex524 or CYP71CU1, expression analysis by qRT-PCR analysis, transcriptome mining to identify biosynthetic genes in the podophyllotoxin pathway, Agrobacterium-mediated functional transient expression in Nicotiana benthamiana | Sinopodophyllum hexandrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-5'-demethoxyyatein + [reduced NADPH-hemoprotein reductase] + O2 | Sinopodophyllum hexandrum | - |
(-)-5'-demethylyatein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinopodophyllum hexandrum | A0A0N9HTU1 | or Sinopodophyllum hexandrum, gene Phex524 or CYP71CU1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-5'-demethoxyyatein + [reduced NADPH-hemoprotein reductase] + O2 | - |
Sinopodophyllum hexandrum | (-)-5'-demethylyatein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the biosynthesis of (-)-4'-demethylepipodophyllotoxin (the etoposide aglycone), a naturally occurring lignan that is the immediate precursor of etoposide and, unlike podophyllotoxin, a potent topoisomerase inhibitor. Biosynthetic pathway of (-)-podophyllotoxin, overview | Sinopodophyllum hexandrum |