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Literature summary for 1.14.14.127 extracted from

  • Jiang, M.; Lue, S.; Zhang, Y.
    Characterization of juvenile hormone related genes regulating cantharidin biosynthesis in Epicauta chinensis (2017), Sci. Rep., 7, 2308 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.127

Cloned(Commentary)

Cloned (Comment) Organism
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis Epicauta chinensis

Protein Variants

Protein Variants Comment Organism
additional information EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection Epicauta chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158 Epicauta chinensis 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2 Epicauta chinensis
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 juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Epicauta chinensis A0A2C9DK12
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Source Tissue

Source Tissue Comment Organism Textmining
head expression level of EcMFE is significantly high in the head of males Epicauta chinensis
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additional information temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating Epicauta chinensis
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
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 Epicauta chinensis juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Subunits

Subunits Comment Organism
? x * 54810, sequence calculation Epicauta chinensis

Synonyms

Synonyms Comment Organism
EcMFE
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 Epicauta chinensis

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 cytochrome P450 conserved site is located at amino acids 432-441 Epicauta chinensis
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Epicauta chinensis

pI Value

Organism Comment pI Value Maximum pI Value
Epicauta chinensis sequence calculation
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 8.29

General Information

General Information Comment Organism
malfunction interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating Epicauta chinensis
metabolism juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III Epicauta chinensis