Cloned (Comment) | Organism |
---|---|
gene CYP716A254, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression in Saccharomyces cerevisiae strain WAT11 results in oxidation of beta-amyrin at the C-28 position to oleanolic acid production | Anemone flaccida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Anemone flaccida | 16020 | - |
microsome | - |
Anemone flaccida | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Anemone flaccida | overall reaction | oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anemone flaccida | I7C6E8 | collected from Tonggubao Village, Machi Town, Changyang County, Hubei Province of China in May of 2014 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
rhizome | - |
Anemone flaccida | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Anemone flaccida | oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
additional information | metabolite identification by mass spectrometry | Anemone flaccida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP716A254 | - |
Anemone flaccida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Anemone flaccida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Anemone flaccida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Anemone flaccida | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Anemone flaccida |
General Information | Comment | Organism |
---|---|---|
metabolism | cytochrome P450 monooxygenase CYP716A254 is involved in oleanane-type triterpenoid saponins biosynthesis, i.e. the biosynthesis of oleanolic acid, which involves cyclization of 2,3-oxidosqualene to the oleanane-type triterpenoid skeleton, followed by a series of oxidation reactions catalyzed by cytochrome P450 monooxygenase (CYP450), Proposed biosynthetic pathway for oleanolic acid in Anemone flaccida, overview | Anemone flaccida |
physiological function | cytochrome P450 monooxygenase CYP716A254 catalyzes the formation of oleanolic acid from beta-amyrin during oleanane-type triterpenoid saponins biosynthesis. Oleanolic acid is natural triterpenoid in plants with diverse biological activities | Anemone flaccida |