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Literature summary for 1.14.14.126 extracted from

  • Carelli, M.; Biazzi, E.; Panara, F.; Tava, A.; Scaramelli, L.; Porceddu, A.; Graham, N.; Odoardi, M.; Piano, E.; Arcioni, S.; May, S.; Scotti, C.; Calderini, O.
    Medicago truncatula CYP716A12 is a multifunctional oxidase involved in the biosynthesis of hemolytic saponins (2011), Plant Cell, 23, 3070-3081.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Medicago truncatula

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Medicago truncatula
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
x * 57000, SDS-PAGE Medicago truncatula

Organism

Organism UniProt Comment Textmining
Medicago truncatula F8J4R7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
flower highest expression of the gene Medicago truncatula
-
leaf significant increase in expression is observed in the reproductive stages, with a maximum expression at flowering Medicago truncatula
-
additional information expression of the gene occurs in all the organs and the developmental stages Medicago truncatula
-
root
-
Medicago truncatula
-
stem
-
Medicago truncatula
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 overall reaction Medicago truncatula oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O CYP716A12 mainly catalyzes the sequential three-step oxidation at the C-28 position necessary to transform beta-amyrin into oleanolic acid ?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
Medicago truncatula oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Subunits

Subunits Comment Organism
? x * 57000, SDS-PAGE Medicago truncatula

Synonyms

Synonyms Comment Organism
CYP716A12
-
Medicago truncatula

General Information

General Information Comment Organism
physiological function enzyme is responsible for an early step in the saponin biosynthetic pathway. Mutants in CYP716A12 are unable to produce hemolytic saponins and only synthetize soyasaponins. CYP716A12 catalyzes the oxidation of beta-amyrin and erythrodiol at the C-28 position, yielding oleanolic acid Medicago truncatula