Cloned (Comment) | Organism |
---|---|
the lovA gene in C-terminal FLAG-tagged form is cloned in the same vector coding cpr (cytochrome P450 oxidoreductase), and both lovA and cpr are co-expressed in Saccharomyces cerevisiae | Aspergillus terreus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus terreus | dihydromonacolin L acid i.e. (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.14.124, monacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin | 3alpha-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | Q9Y7C8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha-hydroxy-3,5-dihydromonacolin L acid | spontaneous | Aspergillus terreus | monacolin L acid + H2O | (1b), spontaneous | ? | |
dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | dihydromonacolin L acid i.e. (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.14.124, monacolin L hydroxylase. The enzyme is involved in the biosynthesis of lovastatin | Aspergillus terreus | 3alpha-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | dihydromonacolin L acid i.e. (3R,5R)-7-[(1S,2S,4aR,6R,8aS)-2,6-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen1yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.14.124, monacolin L hydroxylase | Aspergillus terreus | 3alpha-hydroxy-3,5-dihydromonacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
dihydromonacolin L acid + [reduced NADPH-hemoprotein reductase] + O2 | monacolin L acid i.e. (3R,5R)-7-[(1S,2S,6R,8aR)-2,6-dimethyl-1,2,6,7,8,8a-hexahydronaphthalen-1-yl]-3,5-dihydroxyheptanoate. The enzyme also catalyses the reaction of EC 1.14.13.198, monacolin L hydroxylase | Aspergillus terreus | monacolin L acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LovA | ambiguous | Aspergillus terreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | a heme-thiolate protein | Aspergillus terreus |