Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.120 extracted from

  • Chun, J.H.; Adhikari, P.B.; Park, S.B.; Han, J.Y.; Choi, Y.E.
    Production of the dammarene sapogenin (protopanaxadiol) in transgenic tobacco plants and cultured cells by heterologous expression of PgDDS and CYP716A47 (2015), Plant Cell Rep., 34, 1551-1560 .
    View publication on PubMed
Search for more literature for 1.14.14.120

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP716A47, recombinant expression under control of 35S CaMV promoter in leaves of tobacco via Agrobacterium tumefaciens GV3101 transformation method, RT-PCR expression analysis Panax ginseng

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Panax ginseng 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 Panax ginseng hydroxylation of dammarenediol-II at C-12 protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Panax ginseng H2DH16
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Panax ginseng
-
root
-
 Panax ginseng
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dammarenediol-II + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of dammarenediol-II at C-12 Panax ginseng protopanaxadiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP716A47
-
 Panax ginseng
PPDS
-
 Panax ginseng
protopanaxadiol synthase
-
 Panax ginseng

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Panax ginseng
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Panax ginseng

Expression

Organism Comment Expression
Panax ginseng auxin treatment, particularly 2,4-dichlorophenoxyacetic acid, strongly enhances the transcription of the HMG-Co reductase (HMGR) and squalene epoxidase genes and thus the production of dammarenediol-II and protopanaxadiol (PPD) additional information

General Information

General Information Comment Organism
metabolism the enzyme is part of the biosynthetic pathway for ginsenoside production in Panax ginseng, overview Panax ginseng
physiological function protopanaxadiol (PPD) is an aglycone of dammarene-type ginsenoside and has a wide range of pharmacological activities and high medicinal values. It is synthesized via the hydroxylation of dammarenediol-II (DD) by CYP716A47 enzyme Panax ginseng