KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
8-O-methylsterigmatocystin | in 50 mM phosphate buffer, pH 7.5, at 29°C | Aspergillus parasiticus | |
0.0134 | - |
8-O-methyldihydrosterigmatocystin | in 50 mM phosphate buffer, pH 7.5, at 29°C | Aspergillus parasiticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus flavus | - |
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus parasiticus | - |
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus parasiticus SRRC 163 | - |
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus flavus SRRC 141 | - |
aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus flavus | preferred substrate | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus parasiticus | preferred substrate | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus parasiticus SRRC 163 | preferred substrate | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | Aspergillus flavus SRRC 141 | preferred substrate | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | - |
- |
- |
Aspergillus flavus SRRC 141 | - |
- |
- |
Aspergillus parasiticus | - |
- |
- |
Aspergillus parasiticus SRRC 163 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration | Aspergillus flavus |
ammonium sulfate precipitation, QMA anion exchange column chromatography, and Bio-Gel P-200 gel filtration | Aspergillus parasiticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.23 | - |
enzyme from supernatant, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C | Aspergillus parasiticus |
34.11 | - |
after 148.3fold purification, using 8-O-methylsterigmatocystin as substrate, at pH 7.5 and 29°C | Aspergillus parasiticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Aspergillus flavus | aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Aspergillus parasiticus | aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Aspergillus parasiticus SRRC 163 | aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methyldihydrosterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Aspergillus flavus SRRC 141 | aflatoxin B2 + methanol + CO2 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate | Aspergillus flavus | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate | Aspergillus parasiticus | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate | Aspergillus parasiticus SRRC 163 | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? | |
8-O-methylsterigmatocystin + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate | Aspergillus flavus SRRC 141 | aflatoxin B1 + 2 NADP+ + methanol + [oxidized NADPH-hemoprotein reductase] + H2O + CO2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Aspergillus flavus |
25 | 30 | - |
Aspergillus parasiticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aspergillus flavus |
7.5 | - |
- |
Aspergillus parasiticus |