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Literature summary for 1.14.14.116 extracted from

  • Yu, J.; Chang, P.K.; Cary, J.W.; Bhatnagar, D.; Cleveland, T.E.
    avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus (1997), Appl. Environ. Microbiol., 63, 1349-1356.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.116

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56300
-
 x * 56300, calculated from amino acid sequence Aspergillus parasiticus
56300
-
 x * 56300, calculated from sequence Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin (1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SU1-N3 the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin (1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SU1-N3 the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
averantin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus
-
 5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
averantin + [reduced NADPH-hemoprotein reductase] + O2 Aspergillus parasiticus SRRC 143
-
 5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus parasiticus
-
 ATCC 56775
-
Aspergillus parasiticus Q12732
-
-
Aspergillus parasiticus SRRC 143
-
 ATCC 56775
-
Aspergillus parasiticus SU1-N3 Q12732
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus (1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus (1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus SU1-N3 (1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus SU1-N3 (1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus SU1-N3 (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2 the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin Aspergillus parasiticus SU1-N3 (1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
averantin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus 5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
averantin + [reduced NADPH-hemoprotein reductase] + O2
-
 Aspergillus parasiticus SRRC 143 5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 56300, calculated from amino acid sequence Aspergillus parasiticus
? x * 56300, calculated from sequence Aspergillus parasiticus

Synonyms

Synonyms Comment Organism
avnA
-
 Aspergillus parasiticus
avnA gene name, previously named ord-1 Aspergillus parasiticus
CYP60A1
-
 Aspergillus parasiticus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450 a cytochrome P-450-type monooxygenase Aspergillus parasiticus

General Information

General Information Comment Organism
malfunction disruption of the avnA gene in the wild type aflatoxigenic Aspergillus parasiticus strain SU1-N3 results in a nonaflatoxigenic mutant which accumulates a bright yellow pigment. Norsolorinic acid and averantin are not converted to aflatoxin by the mutant whereas 5'-hydroxyaverantin, averufanin, averufin, versicolorin A, sterigmatocystin, and O-methylsterigmatocystin are converted to aflatoxins Aspergillus parasiticus
metabolism the enzyme is involved in the conversion of averantin to averufin in the aflatoxin biosynthetic pathway Aspergillus parasiticus