Cloned (Comment) | Organism |
---|---|
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels | Platycodon grandiflorus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-16beta, main activity of enzyme CYP716A141 | maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-28, reaction of EC 1.14.13.201 | oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
maniladiol + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-28 | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 | Platycodon grandiflorus | hydroxylation at C-16beta | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Platycodon grandiflorus | A0A1I9Q5Z0 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Platycodon grandiflorus | - |
additional information | transcriptome analysis in different tissues using RNA sequencing technology, overview | Platycodon grandiflorus | - |
petal | - |
Platycodon grandiflorus | - |
root | the enzyme is highly expressed in roots | Platycodon grandiflorus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-16beta, main activity of enzyme CYP716A141 | Platycodon grandiflorus | maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-28, reaction of EC 1.14.13.201 | Platycodon grandiflorus | oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
maniladiol + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-28 | Platycodon grandiflorus | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry | Platycodon grandiflorus | ? | - |
? | |
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 | hydroxylation at C-16beta | Platycodon grandiflorus | cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-amyrin C-16beta oxidase | - |
Platycodon grandiflorus |
C-16beta oxidase | - |
Platycodon grandiflorus |
CYP716A141 | - |
Platycodon grandiflorus |
More | cf. EC 1.14.13.201, beta-amyrin C-28 oxidase | Platycodon grandiflorus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay at | Platycodon grandiflorus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Platycodon grandiflorus | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Platycodon grandiflorus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases | Platycodon grandiflorus |