Literature summary for 1.14.14.113 extracted from

Yu, F.; Okamoto, S.; Harada, H.; Yamasaki, K.; Misawa, N.; Utsumi, R.
Zingiber zerumbet CYP71BA1 catalyzes the conversion of ?-humulene to 8-hydroxy-alpha-humulene in zerumbone biosynthesis (2011), Cell. Mol. Life Sci., 68, 1033-1040.

Search for more literature for 1.14.14.113

Cloned(Commentary)

Cloned (Comment)	Organism
functional co-expression of CYP71BA1 with Arabidopsis NADPH-dependent P450 reductase in Saccharomyces cerevisiae strain WAT11 microsomes, additional cloning of Arabidopsis thaliana ATR2 and Zingiber zerumbet ZSS1 encoding an alpha-humulene synthase	Zingiber zerumbet

Protein Variants

Protein Variants	Comment	Organism
additional information	metabolic engineering: functional co-expression of a gene cluster encoding four enzymes of the mevalonate pathway, i.e. mevalonate kinase, phosphomevalonate kinase, mevalonate diphosphate decarboxylase, and isopentenyl diphosphate isomerase, with CYP71BA1 and ZSS1, encoding alpha-humulene synthase, in Escherichia coli leading to the production of 10-hydroxy-alpha-humulene in the presence of mevalonate, overview	Zingiber zerumbet

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	cytochrome P450 enzyme	Zingiber zerumbet

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]	Zingiber zerumbet	-	10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Zingiber zerumbet	E3W9C4	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
rhizome	CYP71BA1 transcripts are detected almost exclusively in rhizomes, expression pattern of ZSS1 transcripts during rhizome development	Zingiber zerumbet	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]	-	Zingiber zerumbet	10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]	CYP71BA1 is a regiospecific cytochrome P450 enzyme	Zingiber zerumbet	10-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O	GC-MS product analysis	?

Synonyms

Synonyms	Comment	Organism
CYP71BA1	-	Zingiber zerumbet

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Zingiber zerumbet

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Zingiber zerumbet

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Zingiber zerumbet

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the cytochrome P450 monooxygenases	Zingiber zerumbet

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Release 2023.1 (January 2023)