Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.109 extracted from

  • Glawischnig, E.; Grün, S.; Frey, M.; Gierl, A.
    Cytochrome P450 monooxygenases of DIBOA biosynthesis: Specificity and conservation among grasses (1999), Phytochemistry, 50, 925-930.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3) Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
3-hydroxyindolin-2-one native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays
0.036
-
3-hydroxyindolin-2-one recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Zea mays
-
-

Organism

Organism UniProt Comment Textmining
Zea mays
-
hybrid Blizzard
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
recombinant enzyme, at pH 7.5 and 25°C Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyindolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
Zea mays 2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
BX4
-
Zea mays

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Zea mays