Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | NADH dehydrogenase component forming a loose complex with the oxygenating component | Pseudomonas putida | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
additional information | - |
enzyme components form a very loose complex | Pseudomonas putida |
44000 | - |
about, oxygenating component, native PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida | - |
inducible by (+)-camphor | - |
Pseudomonas putida C1 / ATCC 17453 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
both components | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no enzyme assay possible in crude extract due to competition with other enzymes for NADH | Pseudomonas putida |
0.335 | - |
substrate (+)-camphor | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | - |
? | |
(+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida C1 / ATCC 17453 | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | - |
? | |
(+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida | 1,2-campholide + FMN + H2O | - |
? | |
(+)-bornanone + FMNH2 + O2 | (+)-camphor | Pseudomonas putida C1 / ATCC 17453 | 1,2-campholide + FMN + H2O | - |
? | |
additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida | ? | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? | |
additional information | no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 | Pseudomonas putida | ? | - |
? | |
additional information | enzyme is associated with a NADH oxidase | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
additional information | rubredoxin not mentioned | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? | |
additional information | no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 | Pseudomonas putida C1 / ATCC 17453 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme components form a very loose complex | Pseudomonas putida |
More | enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketocamphane 1,2-monooxygenase | - |
Pseudomonas putida |
3,6-diketocamphane 1,6-monooxygenase | different enzyme with similar biochemical properties, immunological cross-reactivity | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | binds reversibly | Pseudomonas putida |