Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.108 extracted from

  • Jones, K.H.; Smith, R.T.; Trudgill, P.W.
    Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453 (1993), J. Gen. Microbiol., 139, 797-805.
    View publication on PubMed
Search for more literature for 1.14.14.108

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm NADH dehydrogenase component forming a loose complex with the oxygenating component Pseudomonas putida 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase Pseudomonas putida
additional information
-
 enzyme components form a very loose complex Pseudomonas putida
44000
-
 about, oxygenating component, native PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida
-
 inducible by (+)-camphor
-
Pseudomonas putida C1 / ATCC 17453
-
-
-

Purification (Commentary)

Purification (Comment) Organism
both components Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no enzyme assay possible in crude extract due to competition with other enzymes for NADH Pseudomonas putida
0.335
-
 substrate (+)-camphor Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O
-
 ?
(+)-bornane-2,5-dione + FMNH2 + O2 i.e. 2,5-diketocamphane Pseudomonas putida C1 / ATCC 17453 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O
-
 ?
(+)-bornanone + FMNH2 + O2 (+)-camphor Pseudomonas putida 1,2-campholide + FMN + H2O
-
 ?
(+)-bornanone + FMNH2 + O2 (+)-camphor Pseudomonas putida C1 / ATCC 17453 1,2-campholide + FMN + H2O
-
 ?
additional information enzyme is associated with a NADH oxidase Pseudomonas putida ?
-
 ?
additional information rubredoxin not mentioned Pseudomonas putida ?
-
 ?
additional information no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 Pseudomonas putida ?
-
 ?
additional information enzyme is associated with a NADH oxidase Pseudomonas putida C1 / ATCC 17453 ?
-
 ?
additional information rubredoxin not mentioned Pseudomonas putida C1 / ATCC 17453 ?
-
 ?
additional information no activity with (-)-camphor and other ketocamphanes that do not possess a keto group at position 2 Pseudomonas putida C1 / ATCC 17453 ?
-
 ?

Subunits

Subunits Comment Organism
More enzyme components form a very loose complex Pseudomonas putida
More enzyme consists of 2 components: 1 oxygenating and 1 NADH dehydrogenase Pseudomonas putida

Synonyms

Synonyms Comment Organism
2,5-diketocamphane 1,2-monooxygenase
-
 Pseudomonas putida
3,6-diketocamphane 1,6-monooxygenase different enzyme with similar biochemical properties, immunological cross-reactivity Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN binds reversibly Pseudomonas putida