Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | boiled cell-free extract, activation, replaces FMN in the assay | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridine | inhibition of the whole enzyme complex, but not of the components alone; reactivation by Fe2+, not by Co2+, Cu2+, Mn2+, Mg2+, Zn2+, Fe3+ | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pseudomonas putida | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | requirement | Pseudomonas putida | |
Fe2+ | no Fe2+ in dehydrogenase | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
wild type strain C1B (PpG1) | - |
Purification (Comment) | Organism |
---|---|
component E1, FMN-reductase | Pseudomonas putida |
component E2, lactonizing enzyme | Pseudomonas putida |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(+)-bornane-2,5-dione + FMNH2 + O2 = (+)-5-oxo-1,2-campholide + FMN + H2O | mechanism | Pseudomonas putida |
Renatured (Comment) | Organism |
---|---|
inactive enzyme after dialysis can be reactivated by Fe2+ at 0.1 mM, Fe3+, Co2+, Zn2+, Mn2+, Mg2+, and Cu2+ are not usefull for reactivation | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.67 | - |
component E2, lactonizing | Pseudomonas putida |
38.3 | - |
component E1, FMN-reductase | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-bornane-2,5-dione + FMNH2 + O2 | i.e. 2,5-diketocamphane | Pseudomonas putida | 3,4,4-trimethyl-5-carboxy-methyl-DELTA2-cyclopentenone + FMN + H2O | i.e. cyclopentenoic acid | ? | |
additional information | rubredoxin not mentioned | Pseudomonas putida | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | activation, 30% activity of FMN | Pseudomonas putida | |
FMN | both enzymatic steps require FMN, not replaceable by GSH, Cys, sodium borohydride or ascorbate | Pseudomonas putida |