Cloned (Comment) | Organism |
---|---|
gene HvKAO1, quantitative RT-PCR enzyme expression analysis | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Hordeum vulgare | overall reaction | gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q9AXH9 | cv. Himalaya | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Hordeum vulgare | - |
seed | - |
Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Hordeum vulgare | gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ent-kaurenoic acid oxidase 1 | UniProt | Hordeum vulgare |
HvKAO1 | - |
Hordeum vulgare |
KAO | - |
Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Hordeum vulgare | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Hordeum vulgare |
Organism | Comment | Expression |
---|---|---|
Hordeum vulgare | diphenyleneiodonium reduces the expression of HvKAO1, about 20% at 1 mM | down |
Hordeum vulgare | exogenous H2O2 promotes the expression of HvKAO1 in barley embryos | up |
General Information | Comment | Organism |
---|---|---|
physiological function | reactive oxygen species (ROS), produced by NADPH oxidase, are involved in gibberellin biosynthesis through the regulation of ent-kaurenoic acid oxidase HvKAO1 and GA3-oxidase HvGA3ox1, relationship between ROS and gibberellin (GA) biosynthesis enzymes, overview | Hordeum vulgare |