Cloned (Comment) | Organism |
---|---|
both genes PsKAO1 and PsKAO2 encode the ent-kaurenoic acid oxidase, expression in Saccharomyces cerevisiae | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 | Pisum sativum | - |
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 | Pisum sativum | - |
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2 | Pisum sativum | the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12 | gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
apical bud | expression of the gene PsKAO1 | Pisum sativum | - |
leaf | expression of the gene PsKAO1 | Pisum sativum | - |
pod | expression of the gene PsKAO1 | Pisum sativum | - |
root | expression of the gene PsKAO1 | Pisum sativum | - |
seed | developing, expression of the gene PsKAO1, the gene PsKAO1 is expressed only in seed | Pisum sativum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pisum sativum | gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12 | Pisum sativum | gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Pisum sativum | ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12 | Pisum sativum | ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2 | the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12 | Pisum sativum | gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | cytochrome P450 protein | Pisum sativum |