BRENDA - Enzyme Database show
show all sequences of 1.14.14.106

Taxol biosynthesis: taxane 13alpha-hydroxylase is a cytochrome P450-dependent monooxygenase

Jennewein, S.; Rithner, C.D.; Williams, R.M.; Croteau, R.B.; Proc. Natl. Acad. Sci. USA 98, 13595-13600 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologous expression in Sf9 insect cells
Taxus cuspidata
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
taxa-4(20),11-dien-5alpha-ol
-
Taxus cuspidata
0.024
-
taxa-4(20),11-dien-5alpha-yl-acetate
-
Taxus cuspidata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus cuspidata
Q8W4T9
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Taxus cuspidata
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
taxa-4(20),11-dien-5alpha-ol + [reduced NADPH-hemoprotein reductase] + O2
-
440249
Taxus cuspidata
taxa-4(20),11-dien-5alpha,13alpha-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
440249
Taxus cuspidata
-
taxa-4(20),11-dien-5alpha-yl-acetate + [reduced NADPH-hemoprotein reductase] + O2
6% of activity with taxa-4(20),11-dien-5alpha-ol
440249
Taxus cuspidata
taxa-4(20),11-dien-5alpha,13alpha-diol-5-acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440249
Taxus cuspidata
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Taxus cuspidata
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologous expression in Sf9 insect cells
Taxus cuspidata
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
taxa-4(20),11-dien-5alpha-ol
-
Taxus cuspidata
0.024
-
taxa-4(20),11-dien-5alpha-yl-acetate
-
Taxus cuspidata
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Taxus cuspidata
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
taxa-4(20),11-dien-5alpha-ol + [reduced NADPH-hemoprotein reductase] + O2
-
440249
Taxus cuspidata
taxa-4(20),11-dien-5alpha,13alpha-diol + [oxidized NADPH-hemoprotein reductase] + H2O
-
440249
Taxus cuspidata
-
taxa-4(20),11-dien-5alpha-yl-acetate + [reduced NADPH-hemoprotein reductase] + O2
6% of activity with taxa-4(20),11-dien-5alpha-ol
440249
Taxus cuspidata
taxa-4(20),11-dien-5alpha,13alpha-diol-5-acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
440249
Taxus cuspidata
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Taxus cuspidata
Other publictions for EC 1.14.14.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672704
DeJong
Genetic engineering of taxol b ...
Taxus sp.
Biotechnol. Bioeng.
93
212-224
2006
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
672699
Jennewein
Coexpression in yeast of Taxus ...
Taxus cuspidata
Biotechnol. Bioeng.
89
588-598
2005
-
-
1
-
-
-
-
-
2
1
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
440248
Wheeler
Taxol biosynthesis: differenti ...
Taxus cuspidata
Arch. Biochem. Biophys.
390
265-278
2001
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
440249
Jennewein
Taxol biosynthesis: taxane 13a ...
Taxus cuspidata
Proc. Natl. Acad. Sci. USA
98
13595-13600
2001
-
-
1
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-