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Literature summary for 1.14.14.103 extracted from

  • Besseau, S.; Kellner, F.; Lanoue, A.; Thamm, A.M.; Salim, V.; Schneider, B.; Geu-Flores, F.; Hoefer, R.; Guirimand, G.; Guihur, A.; Oudin, A.; Glevarec, G.; Foureau, E.; Papon, N.; Clastre, M.; Giglioli-Guivarch, N.; St-Pierre, B.; Werck-Reichhart, D.; Burlat, V.; De Luca, V.; OConnor, S.E.; Cou, C.o.u.r.
    A pair of tabersonine 16-hydroxylases initiates the synthesis of vindoline in an organ-dependent manner in Catharanthus roseus (2013), Plant Physiol., 163, 1792-1803.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae WAT11 cells Catharanthus roseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00007
-
 tabersonine isoform T16H2, at pH 8.0 and 30°C Catharanthus roseus
0.00035
-
 tabersonine isoform T16H1, at pH 8.0 and 30°C Catharanthus roseus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum CYP71D351 (isoform T16H2) Catharanthus roseus 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus low activity 2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2 Catharanthus roseus low activity 2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information Catharanthus roseus no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine ?
-
 ?
tabersonine + NADPH + H+ + O2 Catharanthus roseus highest activity 16-hydroxytabersonine + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Catharanthus roseus C0KYN4 CYP71D12; cultivar Pacifica Pink
-

Source Tissue

Source Tissue Comment Organism Textmining
flower isoform T16H1, CYP71D351 Catharanthus roseus
-
leaf epidermis highest expression of isoform T16H2, CYP71D12 in young leaves Catharanthus roseus
-
additional information CYP71D12 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds Catharanthus roseus
-
additional information CYP71D351 transcripts are barely detectable or undetectable in roots, internodes, young and mature leaves, and flower buds Catharanthus roseus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydro-3-hydroxytabersonine + [reduced NADPH-hemoprotein reductase] + O2 low activity Catharanthus roseus 2,3-dihydro-3,16-dihydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
2,3-dihydrotabersonine + [reduced NADPH-hemoprotein reductase] + O2 low activity Catharanthus roseus 2,3-dihydro-16-hydroxytabersonine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline, and catharanthine Catharanthus roseus ?
-
 ?
tabersonine + NADPH + H+ + O2 highest activity Catharanthus roseus 16-hydroxytabersonine + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
CYP71D12 isoform T16H2 Catharanthus roseus
CYP71D351 isoform T16H1 Catharanthus roseus
T16H1 isoform Catharanthus roseus
T16H2 isoform Catharanthus roseus

General Information

General Information Comment Organism
metabolism CYP71D12 (isoform T16H2) plays a role in the biosynthesis of vindoline in flowers Catharanthus roseus
metabolism CYP71D351 (isoform T16H2) has a major role in the biosynthesis of vindoline in leaves Catharanthus roseus