Purification and characterization of EDTA monooxygenase from the EDTA-degrading bacterium BNC1
Payne, J.W.; Bolton, H.; Campbell, J.A.; Xun, L.; J. Bacteriol. 180, 3823-3827 (1998) 
Data extracted from this reference:
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
EDTA monooxygenase degrades nitrilotriacetate in the absence or presence of Mg2+
Chelativorans sp. BNC1
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Chelativorans sp. BNC1
Organism
Organism
UniProt
Commentary
Textmining
Chelativorans sp. BNC1
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
ammonium sulfate precipitation, Blue 3GA agarose column chromatography, MonoQ HR 5/5 column chromatography, and hydroxyapatite column chromatography
Chelativorans sp. BNC1
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
EDTA + O2 + FMNH2 + H+
-
717720
Chelativorans sp. BNC1
ethylenediaminetriacetate + glyoxylate + H2O + FMN
-
-
-
?
nitrilotriacetate + O2 + FMNH2 + H+
-
717720
Chelativorans sp. BNC1
iminodiacetate + glyoxylate + H2O + FMN
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Chelativorans sp. BNC1
Synonyms
Synonyms
Commentary
Organism
EDTA monooxygenase
-
Chelativorans sp. BNC1
NTA-MO
-
Chelativorans sp. BNC1
Cofactor
Cofactor
Commentary
Organism
Structure
FMNH2
-
Chelativorans sp. BNC1
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMNH2
-
Chelativorans sp. BNC1
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
EDTA monooxygenase degrades nitrilotriacetate in the absence or presence of Mg2+
Chelativorans sp. BNC1
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
gel filtration
Chelativorans sp. BNC1
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, Blue 3GA agarose column chromatography, MonoQ HR 5/5 column chromatography, and hydroxyapatite column chromatography
Chelativorans sp. BNC1
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
EDTA + O2 + FMNH2 + H+
-
717720
Chelativorans sp. BNC1
ethylenediaminetriacetate + glyoxylate + H2O + FMN
-
-
-
?
nitrilotriacetate + O2 + FMNH2 + H+
-
717720
Chelativorans sp. BNC1
iminodiacetate + glyoxylate + H2O + FMN
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 45000, SDS-PAGE
Chelativorans sp. BNC1
Other publictions for EC 1.14.14.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728259
Van Hamme
Genomic and proteomic characte ...
Gordonia sp., Gordonia sp. NB4-1Y
Microbiology
159
1618-1628
2013
-
-
-
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-
-
-
-
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4
-
2
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4
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1
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1
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1
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4
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4
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
717038
Zhang
Structure of nitrilotriacetate ...
Mycolicibacterium thermoresistibile
Acta Crystallogr. Sect. F
67
1100-1105
2011
-
-
1
1
-
-
-
-
-
-
-
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-
6
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1
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1
1
2
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1
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1
1
1
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1
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1
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
717028
Kim
Crystallization and initial cr ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
62
1141-1143
2006
-
-
-
1
-
-
-
-
-
1
2
-
-
2
-
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1
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1
1
2
-
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-
1
-
-
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-
-
1
1
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717178
Ternan
Iminodiacetate and nitrilotria ...
Kluyveromyces marxianus, Kluyveromyces marxianus IMB3
Biochem. Biophys. Res. Commun.
290
802-805
2002
-
-
-
-
-
1
-
-
-
1
-
-
-
4
-
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-
-
-
1
-
2
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717720
Payne
Purification and characterizat ...
Chelativorans sp. BNC1
J. Bacteriol.
180
3823-3827
1998
-
-
-
-
-
-
-
-
-
1
1
-
-
4
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
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-
-
-
1
-
-
-
-
-
-
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-
1
1
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-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717717
Xu
Cloning, sequencing, and analy ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
179
1112-1116
1997
-
-
1
-
-
-
-
-
-
1
1
-
-
4
-
-
-
-
-
-
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4
1
1
-
-
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-
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-
-
1
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-
-
1
1
-
-
-
-
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1
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717116
Bally
Dynamics of substrate consumpt ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Appl. Environ. Microbiol.
62
133-140
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
717545
Xun
-
Degradation of metal - nitrilo ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Environ. Sci. Technol.
30
1752-1755
1996
-
-
-
-
-
-
2
7
-
7
-
-
-
5
-
-
1
-
-
-
-
1
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
7
-
7
-
-
-
-
-
1
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717715
Knobel
Cloning and characterization o ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
178
6123-6132
1996
-
-
1
-
-
-
-
-
-
1
1
-
-
6
-
-
-
-
-
-
1
-
2
1
3
-
-
-
-
-
-
-
1
-
1
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-
-
1
1
-
-
-
-
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1
1
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1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
718086
Bally
Growth and regulation of enzym ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Microbiology
140
1927-1936
1994
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1
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7
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2
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1
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1
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1
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1
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2
-
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-
-
-
-
1
-
-
1
-
-
715329
Uetz
Purification and characterizat ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
174
1179-1188
1992
3
-
-
-
-
-
6
2
-
4
1
-
-
6
-
-
1
-
-
-
-
5
4
1
3
1
-
-
-
2
1
-
2
-
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3
-
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2
-
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6
-
2
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4
1
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1
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5
4
1
1
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2
1
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