BRENDA - Enzyme Database show
show all sequences of 1.14.14.10

Cloning, sequencing, and analysis of a gene cluster from Chelatobacter heintzii ATCC 29600 encoding nitrilotriacetate monooxygenase and NADH:flavin mononucleotide oxidoreductase

Xu, Y.; Mortimer, M.W.; Fisher, T.S.; Kahn, M.L.; Brockman, F.J.; Xun, L.; J. Bacteriol. 179, 1112-1116 (1997)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli JM105 cells
Aminobacter aminovorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Aminobacter aminovorans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34500
-
x * 34500, calculated from amino acid sequence
Aminobacter aminovorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aminobacter aminovorans
P54989
component A of nitrilotriacetate monooxygenase
-
Aminobacter aminovorans ATCC 29600
P54989
component A of nitrilotriacetate monooxygenase
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the NTA monooxygenase activity requires two component proteins, component A and component B. Component A is an NTA monooxygenase that uses FMNH2 and O2 to oxidize nitrilotriacetate, and component B is an NADH:FMN oxidoreductase that provides FMNH2 for nitrilotriacetate oxidation
717717
Aminobacter aminovorans
?
-
-
-
-
additional information
the NTA monooxygenase activity requires two component proteins, component A and component B. Component A is an NTA monooxygenase that uses FMNH2 and O2 to oxidize nitrilotriacetate, and component B is an NADH:FMN oxidoreductase that provides FMNH2 for nitrilotriacetate oxidation
717717
Aminobacter aminovorans ATCC 29600
?
-
-
-
-
nitrilotriacetate + FMNH2 + H+ + O2
-
717717
Aminobacter aminovorans
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
nitrilotriacetate + FMNH2 + H+ + O2
-
717717
Aminobacter aminovorans ATCC 29600
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 34500, calculated from amino acid sequence
Aminobacter aminovorans
Cofactor
Cofactor
Commentary
Organism
Structure
FMNH2
-
Aminobacter aminovorans
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli JM105 cells
Aminobacter aminovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMNH2
-
Aminobacter aminovorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Aminobacter aminovorans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34500
-
x * 34500, calculated from amino acid sequence
Aminobacter aminovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the NTA monooxygenase activity requires two component proteins, component A and component B. Component A is an NTA monooxygenase that uses FMNH2 and O2 to oxidize nitrilotriacetate, and component B is an NADH:FMN oxidoreductase that provides FMNH2 for nitrilotriacetate oxidation
717717
Aminobacter aminovorans
?
-
-
-
-
additional information
the NTA monooxygenase activity requires two component proteins, component A and component B. Component A is an NTA monooxygenase that uses FMNH2 and O2 to oxidize nitrilotriacetate, and component B is an NADH:FMN oxidoreductase that provides FMNH2 for nitrilotriacetate oxidation
717717
Aminobacter aminovorans ATCC 29600
?
-
-
-
-
nitrilotriacetate + FMNH2 + H+ + O2
-
717717
Aminobacter aminovorans
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
nitrilotriacetate + FMNH2 + H+ + O2
-
717717
Aminobacter aminovorans ATCC 29600
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 34500, calculated from amino acid sequence
Aminobacter aminovorans
Other publictions for EC 1.14.14.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728259
Van Hamme
Genomic and proteomic characte ...
Gordonia sp., Gordonia sp. NB4-1Y
Microbiology
159
1618-1628
2013
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-
-
-
-
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4
-
2
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4
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1
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1
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4
-
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4
-
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-
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-
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-
-
-
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-
717038
Zhang
Structure of nitrilotriacetate ...
Mycobacterium thermoresistibile
Acta Crystallogr. Sect. F
67
1100-1105
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
1
1
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-
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-
1
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1
1
1
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1
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1
1
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-
717028
Kim
Crystallization and initial cr ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
62
1141-1143
2006
-
-
-
1
-
-
-
-
-
1
2
-
-
2
-
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1
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1
1
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1
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1
1
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1
2
-
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-
-
1
-
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-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717178
Ternan
Iminodiacetate and nitrilotria ...
Kluyveromyces marxianus, Kluyveromyces marxianus IMB3
Biochem. Biophys. Res. Commun.
290
802-805
2002
-
-
-
-
-
1
-
-
-
1
-
-
-
4
-
-
-
-
-
1
-
2
2
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
1
-
-
1
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-
1
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1
-
2
2
-
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-
-
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-
-
-
-
717720
Payne
Purification and characterizat ...
Chelativorans sp.
J. Bacteriol.
180
3823-3827
1998
-
-
-
-
-
-
-
-
-
1
1
-
-
3
-
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1
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2
1
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1
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1
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1
1
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1
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2
1
-
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-
-
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-
-
-
717717
Xu
Cloning, sequencing, and analy ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
179
1112-1116
1997
-
-
1
-
-
-
-
-
-
1
1
-
-
5
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717116
Bally
Dynamics of substrate consumpt ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Appl. Environ. Microbiol.
62
133-140
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
717545
Xun
-
Degradation of metal - nitrilo ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Environ. Sci. Technol.
30
1752-1755
1996
-
-
-
-
-
-
2
7
-
7
-
-
-
5
-
-
1
-
-
-
-
1
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
7
-
7
-
-
-
-
-
1
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
717715
Knobel
Cloning and characterization o ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
178
6123-6132
1996
-
-
1
-
-
-
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1
1
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6
-
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-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
718086
Bally
Growth and regulation of enzym ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Microbiology
140
1927-1936
1994
-
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-
-
-
-
-
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1
-
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6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
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-
1
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1
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-
-
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-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
715329
Uetz
Purification and characterizat ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
174
1179-1188
1992
3
-
-
-
-
-
6
2
-
4
1
-
-
5
-
-
1
-
-
-
-
5
4
1
1
-
-
-
2
1
-
2
-
-
-
3
-
-
2
-
-
-
-
6
-
2
-
4
1
-
-
-
-
1
-
-
-
5
4
1
1
-
-
-
2
1
-
-
-
-
-
-
-
-