Data extracted from this reference:
Cloned(Commentary)
expressed in Escherichia coli DH5alpha cells
Aminobacter aminovorans
Metals/Ions
Mg2+
required
Aminobacter aminovorans
Molecular Weight [Da]
50500
x * 50500, calculated from amino acid sequence
Aminobacter aminovorans
Organism
Aminobacter aminovorans
P54989
component A of NTA monooxygenase
Aminobacter aminovorans ATCC 29600
P54989
component A of NTA monooxygenase
Specific Activity [micromol/min/mg]
0.076
in 30 mM Tris-HCl (pH 8.0), 10 mM FMN, and 2 mM MgCl2, temperature not specified in the publication
Aminobacter aminovorans
Substrates and Products (Substrate)
nitrilotriacetate + FMNH2 + H+ + O2
717715
Aminobacter aminovorans
iminodiacetate + glyoxylate + FMN + H2O
ir
nitrilotriacetate + FMNH2 + H+ + O2
717715
Aminobacter aminovorans ATCC 29600
iminodiacetate + glyoxylate + FMN + H2O
ir
Subunits
?
x * 50500, calculated from amino acid sequence
Aminobacter aminovorans
Synonyms
NTA monooxygenase
Aminobacter aminovorans
NTA-MO
Aminobacter aminovorans
NtaA
component A of NTA monooxygenase
Aminobacter aminovorans
Cofactor
FMNH2
Aminobacter aminovorans
pI Value
Aminobacter aminovorans
calculated from amino acid sequence
6.5
Cloned(Commentary) (protein specific)
expressed in Escherichia coli DH5alpha cells
Aminobacter aminovorans
Cofactor (protein specific)
FMNH2
Aminobacter aminovorans
Metals/Ions (protein specific)
Mg2+
required
Aminobacter aminovorans
Molecular Weight [Da] (protein specific)
50500
x * 50500, calculated from amino acid sequence
Aminobacter aminovorans
Specific Activity [micromol/min/mg] (protein specific)
0.076
in 30 mM Tris-HCl (pH 8.0), 10 mM FMN, and 2 mM MgCl2, temperature not specified in the publication
Aminobacter aminovorans
Substrates and Products (Substrate) (protein specific)
nitrilotriacetate + FMNH2 + H+ + O2
717715
Aminobacter aminovorans
iminodiacetate + glyoxylate + FMN + H2O
ir
nitrilotriacetate + FMNH2 + H+ + O2
717715
Aminobacter aminovorans ATCC 29600
iminodiacetate + glyoxylate + FMN + H2O
ir
Subunits (protein specific)
?
x * 50500, calculated from amino acid sequence
Aminobacter aminovorans
pI Value (protein specific)
Aminobacter aminovorans
calculated from amino acid sequence
6.5
Other publictions for EC 1.14.14.10
728259
Van Hamme
Genomic and proteomic characte ...
Gordonia sp., Gordonia sp. NB4-1Y
Microbiology
159
1618-1628
2013
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
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-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717038
Zhang
Structure of nitrilotriacetate ...
Mycolicibacterium thermoresistibile
Acta Crystallogr. Sect. F
67
1100-1105
2011
-
-
1
1
-
-
-
-
-
-
-
-
-
11
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
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-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717028
Kim
Crystallization and initial cr ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
62
1141-1143
2006
-
-
-
1
-
-
-
-
-
1
2
-
-
4
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717178
Ternan
Iminodiacetate and nitrilotria ...
Kluyveromyces marxianus, Kluyveromyces marxianus IMB3
Biochem. Biophys. Res. Commun.
290
802-805
2002
-
-
-
-
-
1
-
-
-
1
-
-
-
5
-
-
-
-
-
1
-
2
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717720
Payne
Purification and characterizat ...
Chelativorans sp. BNC1
J. Bacteriol.
180
3823-3827
1998
-
-
-
-
-
-
-
-
-
1
1
-
-
5
-
-
1
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717717
Xu
Cloning, sequencing, and analy ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
179
1112-1116
1997
-
-
1
-
-
-
-
-
-
1
1
-
-
7
-
-
-
-
-
-
-
-
4
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717116
Bally
Dynamics of substrate consumpt ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Appl. Environ. Microbiol.
62
133-140
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
717545
Xun
-
Degradation of metal - nitrilo ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Environ. Sci. Technol.
30
1752-1755
1996
-
-
-
-
-
-
2
7
-
7
-
-
-
5
-
-
1
-
-
-
-
1
4
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
7
-
7
-
-
-
-
-
1
-
-
-
1
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
717715
Knobel
Cloning and characterization o ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
178
6123-6132
1996
-
-
1
-
-
-
-
-
-
1
1
-
-
9
-
-
-
-
-
-
1
-
2
1
3
-
-
-
-
-
-
-
1
-
1
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-
1
1
-
-
-
-
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-
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-
1
1
-
-
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-
-
1
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
718086
Bally
Growth and regulation of enzym ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Microbiology
140
1927-1936
1994
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1
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7
-
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-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
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-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
715329
Uetz
Purification and characterizat ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
174
1179-1188
1992
3
-
-
-
-
-
6
2
-
5
1
-
-
7
-
-
1
-
-
-
-
5
4
1
3
1
-
-
-
2
1
-
2
-
-
-
3
-
-
2
-
-
-
-
6
-
2
-
5
1
-
-
-
-
1
-
-
-
5
4
1
1
-
-
-
2
1
-
-
-
-
-
-
-
-