BRENDA - Enzyme Database show
show all sequences of 1.14.14.10

Degradation of metal - nitrilotriacetate complexes by nitrilotriacetate monooxygenase

Xun, L.; Reeder, R.; Plymale, A.; Girvin, D.; Bolton Jr., H.; Environ. Sci. Technol. 30, 1752-1755 (1996)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Ni2+
ionic or uncomplexed Ni2+ (2 mM) is inhibitory to the enzyme
Aminobacter aminovorans
Zn2+
ionic or uncomplexed Zn2+ (2 mM) is inhibitory to the enzyme
Aminobacter aminovorans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0317
-
nitrilotriacetate
with equimolar Ni2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0367
-
nitrilotriacetate
with equimolar Co2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0404
-
nitrilotriacetate
with equimolar Mg2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.043
-
nitrilotriacetate
with 2 mM Mg2+and varying nitrilotriacetate concentrations, in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0576
-
nitrilotriacetate
with equimolar Zn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0905
-
nitrilotriacetate
with equimolar Mn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0968
-
nitrilotriacetate
with equimolar Fe2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Al3+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Co2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Cu2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Fe2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Mg2+
the enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate
Aminobacter aminovorans
Ni2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Zn2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aminobacter aminovorans
-
-
-
Aminobacter aminovorans ATCC 29600
-
-
-
Purification (Commentary)
Commentary
Organism
partially purified by ammonium sulfate precipitation and phenyl agarose column chromatography
Aminobacter aminovorans
Storage Stability
Storage Stability
Organism
-80°C, partially purified, dialyzed enzyme, 3 months, no loss of activity
Aminobacter aminovorans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate
717545
Aminobacter aminovorans
?
-
-
-
-
additional information
nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate
717545
Aminobacter aminovorans ATCC 29600
?
-
-
-
-
nitrilotriacetate + FMNH2 + H+ + O2
nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate
717545
Aminobacter aminovorans
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
nitrilotriacetate + FMNH2 + H+ + O2
nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate
717545
Aminobacter aminovorans ATCC 29600
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
FMNH2
-
Aminobacter aminovorans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMNH2
-
Aminobacter aminovorans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ni2+
ionic or uncomplexed Ni2+ (2 mM) is inhibitory to the enzyme
Aminobacter aminovorans
Zn2+
ionic or uncomplexed Zn2+ (2 mM) is inhibitory to the enzyme
Aminobacter aminovorans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0317
-
nitrilotriacetate
with equimolar Ni2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0367
-
nitrilotriacetate
with equimolar Co2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0404
-
nitrilotriacetate
with equimolar Mg2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.043
-
nitrilotriacetate
with 2 mM Mg2+and varying nitrilotriacetate concentrations, in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0576
-
nitrilotriacetate
with equimolar Zn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0905
-
nitrilotriacetate
with equimolar Mn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
0.0968
-
nitrilotriacetate
with equimolar Fe2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C
Aminobacter aminovorans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Al3+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Co2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Cu2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Fe2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Mg2+
the enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate
Aminobacter aminovorans
Ni2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Zn2+
nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+
Aminobacter aminovorans
Purification (Commentary) (protein specific)
Commentary
Organism
partially purified by ammonium sulfate precipitation and phenyl agarose column chromatography
Aminobacter aminovorans
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, partially purified, dialyzed enzyme, 3 months, no loss of activity
Aminobacter aminovorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate
717545
Aminobacter aminovorans
?
-
-
-
-
additional information
nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate
717545
Aminobacter aminovorans ATCC 29600
?
-
-
-
-
nitrilotriacetate + FMNH2 + H+ + O2
nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate
717545
Aminobacter aminovorans
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
nitrilotriacetate + FMNH2 + H+ + O2
nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate
717545
Aminobacter aminovorans ATCC 29600
iminodiacetate + glyoxylate + FMN + H2O
-
-
-
?
Other publictions for EC 1.14.14.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728259
Van Hamme
Genomic and proteomic characte ...
Gordonia sp., Gordonia sp. NB4-1Y
Microbiology
159
1618-1628
2013
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717038
Zhang
Structure of nitrilotriacetate ...
Mycobacterium thermoresistibile
Acta Crystallogr. Sect. F
67
1100-1105
2011
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1
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717028
Kim
Crystallization and initial cr ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
62
1141-1143
2006
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1
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717178
Ternan
Iminodiacetate and nitrilotria ...
Kluyveromyces marxianus, Kluyveromyces marxianus IMB3
Biochem. Biophys. Res. Commun.
290
802-805
2002
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717720
Payne
Purification and characterizat ...
Chelativorans sp.
J. Bacteriol.
180
3823-3827
1998
-
-
-
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1
1
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3
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717717
Xu
Cloning, sequencing, and analy ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
179
1112-1116
1997
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1
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1
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5
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1
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717116
Bally
Dynamics of substrate consumpt ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Appl. Environ. Microbiol.
62
133-140
1996
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6
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717545
Xun
-
Degradation of metal - nitrilo ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Environ. Sci. Technol.
30
1752-1755
1996
-
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2
7
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7
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5
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717715
Knobel
Cloning and characterization o ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
178
6123-6132
1996
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718086
Bally
Growth and regulation of enzym ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Microbiology
140
1927-1936
1994
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715329
Uetz
Purification and characterizat ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
174
1179-1188
1992
3
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