Data extracted from this reference:
Cloned(Commentary)
expressed in Escherichia coli BL21(DE3) Rosetta cells
Mycolicibacterium thermoresistibile
Crystallization (Commentary)
sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M MES pH 6.0 and 20% PEG 6000
Mycolicibacterium thermoresistibile
Organism
Mycolicibacterium thermoresistibile
E5Q9D7
Purification (Commentary)
HisTrap FF column chromatography and Superdex 75 gel filtration
Mycolicibacterium thermoresistibile
Substrates and Products (Substrate)
nitrilotriacetate + FMNH2 + H+ + O2
717038
Mycolicibacterium thermoresistibile
iminodiacetate + glyoxylate + FMN + H2O
?
Subunits
homodimer
x-ray crystallography
Mycolicibacterium thermoresistibile
Synonyms
nitrilotriacetate monooxygenase component B
Mycolicibacterium thermoresistibile
NTA-MoB
Mycolicibacterium thermoresistibile
Cofactor
FMNH2
Mycolicibacterium thermoresistibile
Cloned(Commentary) (protein specific)
expressed in Escherichia coli BL21(DE3) Rosetta cells
Mycolicibacterium thermoresistibile
Cofactor (protein specific)
FMNH2
Mycolicibacterium thermoresistibile
Crystallization (Commentary) (protein specific)
sitting drop vapor diffusion method, using 0.2 M magnesium chloride, 0.1 M MES pH 6.0 and 20% PEG 6000
Mycolicibacterium thermoresistibile
Purification (Commentary) (protein specific)
HisTrap FF column chromatography and Superdex 75 gel filtration
Mycolicibacterium thermoresistibile
Substrates and Products (Substrate) (protein specific)
nitrilotriacetate + FMNH2 + H+ + O2
717038
Mycolicibacterium thermoresistibile
iminodiacetate + glyoxylate + FMN + H2O
?
Subunits (protein specific)
homodimer
x-ray crystallography
Mycolicibacterium thermoresistibile
Other publictions for EC 1.14.14.10
728259
Van Hamme
Genomic and proteomic characte ...
Gordonia sp., Gordonia sp. NB4-1Y
Microbiology
159
1618-1628
2013
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4
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2
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4
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717038
Zhang
Structure of nitrilotriacetate ...
Mycolicibacterium thermoresistibile
Acta Crystallogr. Sect. F
67
1100-1105
2011
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1
1
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11
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717028
Kim
Crystallization and initial cr ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
62
1141-1143
2006
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1
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1
2
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4
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717178
Ternan
Iminodiacetate and nitrilotria ...
Kluyveromyces marxianus, Kluyveromyces marxianus IMB3
Biochem. Biophys. Res. Commun.
290
802-805
2002
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1
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5
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717720
Payne
Purification and characterizat ...
Chelativorans sp. BNC1
J. Bacteriol.
180
3823-3827
1998
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1
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5
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1
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717717
Xu
Cloning, sequencing, and analy ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
179
1112-1116
1997
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1
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1
1
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7
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4
1
1
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1
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1
1
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1
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4
1
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717116
Bally
Dynamics of substrate consumpt ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Appl. Environ. Microbiol.
62
133-140
1996
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7
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2
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2
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1
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1
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2
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2
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2
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717545
Xun
-
Degradation of metal - nitrilo ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Environ. Sci. Technol.
30
1752-1755
1996
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2
7
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7
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5
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1
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1
4
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1
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1
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1
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7
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7
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1
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1
4
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717715
Knobel
Cloning and characterization o ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
178
6123-6132
1996
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1
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1
1
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9
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1
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2
1
3
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1
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1
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1
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1
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1
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1
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718086
Bally
Growth and regulation of enzym ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
Microbiology
140
1927-1936
1994
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7
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715329
Uetz
Purification and characterizat ...
Aminobacter aminovorans, Aminobacter aminovorans ATCC 29600
J. Bacteriol.
174
1179-1188
1992
3
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6
2
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1
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7
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4
1
3
1
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1
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3
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2
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6
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2
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5
1
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1
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5
4
1
1
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2
1
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