Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris strain KM71H | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
W129A/L324G | the mutant shows complete loss of activity toward pyrene and retains partial oxidation activity (32%) toward phenanthrene | Phanerodontia chrysosporium |
W129F/L324F | the mutant shows complete loss of activity toward both phenanthrene and pyrene | Phanerodontia chrysosporium |
W129F/L324G | the mutant shows complete loss of activity toward pyrene and retains partial oxidation activity (15%) toward phenanthrene | Phanerodontia chrysosporium |
W129L/L324F | the mutant shows greater oxidation of phenanthrene and pyrene compared with the wild type enzyme | Phanerodontia chrysosporium |
W129L/L324G | the mutant shows complete loss of activity toward both phenanthrene and pyrene | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenanthrene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Phanerodontia chrysosporium | ? | - |
? | |
pyrene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Phanerodontia chrysosporium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP5136A3 | - |
Phanerodontia chrysosporium |
cytochrome P450 monooxygenase | - |
Phanerodontia chrysosporium |