Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Oryctolagus cuniculus | 5783 | - |
microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme protein | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
CYP1A2 with NADPH-cytochrome P450 reductase preparation of endoplasmic reticulum membranes from microsomes, formation into lipid vesicles. the lipid composition of purified lipid vesicles affects CYP1A2 substrate metabolism and NADPH-cytochrome P450 reductase-CYP1A2 binding | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-ethoxy-4-trifluoromethylcoumarin + [reduced NADPH-hemoprotein reductase] + O2 | CYP1A2 cooperates with NADPH-cytochrome P450 reductase, which provides NADPH for the reaction. Interaction with their redox partner, NADPH-cytochrome P450 reductase, i.e. CPR, in a 1:1 molar ratio | Oryctolagus cuniculus | 7-hydroxy-4-trifluoromethylcoumarin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
7-ethoxyresorufin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryctolagus cuniculus | resorufin + ethanol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | CYP1A2 cooperates with NADPH-cytochrome P450 reductase, CPR. Differential sensitivity of CPR binding to CYP1A2 depending on the substrate present, overview | Oryctolagus cuniculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP1A2 | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Oryctolagus cuniculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Oryctolagus cuniculus | |
NADPH | - |
Oryctolagus cuniculus |