Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, gel filtration | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor | 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + 11,12-epoxyeicosa-5,8,14-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | reaction products generated by isoform CYP102B1 | ? | |
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor A3(2) | 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + 11,12-epoxyeicosa-5,8,14-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | reaction products generated by isoform CYP102B1 | ? | |
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor | 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | reaction products generated by isoform CYP102A1 | ? | |
arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor A3(2) | 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O | reaction products generated by isoform CYP102A1 | ? | |
additional information | palmitate, stearate, and oleate show negligible binding to isoform CYP102B1, in contrast to CYP102A1 | Streptomyces coelicolor | ? | - |
? | |
additional information | palmitate, stearate, and oleate show negligible binding to isoform CYP102B1, in contrast to CYP102A1 | Streptomyces coelicolor A3(2) | ? | - |
? | |
oleic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
oleic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor A3(2) | ? | - |
? | |
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor A3(2) | ? | - |
? | |
stearic acid + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces coelicolor | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP102 | - |
Streptomyces coelicolor |
CYP102A1 | isoform, binds arachidonate more tightly than isoform CYP102B1 | Streptomyces coelicolor |
CYP102B1 | isoform | Streptomyces coelicolor |
fatty acid hydroxylase | - |
Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Streptomyces coelicolor | |
NADPH | - |
Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform CYP102B1 is not required for normal cell growth and secondary metabolite productio | Streptomyces coelicolor |