Literature summary for 1.14.14.1 extracted from

Roccatano, D.; Wong, T.S.; Schwaneberg, U.; Zacharias, M.
Toward understanding the inactivation mechanism of monooxygenase P450 BM-3 by organic cosolvents: a molecular dynamics simulation study (2006), Biopolymers, 83, 467-476.

Search for more literature for 1.14.14.1

Application

Application	Comment	Organism
additional information	the enzyme is a target for improving the catalytic performance of P450 BM-3 toward nonnatural substrates of industrial importance in the presence of organic solvents or cosolvents for industrial applications	Priestia megaterium

Protein Variants

Protein Variants	Comment	Organism
F87A	site-directed mutagenesis, the mutant exhibits an altered regioselectivity and substrate specificity compared with wild-type, it has lower tolerance toward DMSO	Priestia megaterium
additional information	the enzyme is a target for improving the catalytic performance of P450 BM-3 toward nonnatural substrates of industrial importance in the presence of organic solvents or cosolvents for industrial applications	Priestia megaterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	in a catalytic cytochrome P450 ferric heme center	Priestia megaterium

Organic Solvent Stability

Organic Solvent	Comment	Organism
DMSO	inactivation mechanism, the mutant F87A is more sensitive to DMSO than the wild-type enzyme due to the absence of the phenyl ring in the mutant which promotes interactions of the DMSO molecule with the heme iron resulting in water displacement by DMSO at the catalytic heme center, box by stacking equilibrated boxes of solvent molecules to, modeling of DMSO in the active site channel, overview	Priestia megaterium
additional information	inactivation mechanism of monooxygenase P450 BM-3 by organic cosolvents: a molecular dynamics simulation study using the X-ray P450 BM-3 crystal structure, PDB code 1BU7	Priestia megaterium

Organism

Organism	UniProt	Comment	Textmining
Priestia megaterium	P14779	-	-

Subunits

Subunits	Comment	Organism
More	structure alteration due to organic solvents, analysis by molecular dynamics simulation study using the X-ray P450 BM-3 crystal structure, PDB code 1BU7	Priestia megaterium

Synonyms

Synonyms	Comment	Organism
monooxygenase P450 BM-3	-	Priestia megaterium

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Release 2023.1 (January 2023)