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Literature summary for 1.14.13.B28 extracted from
- Characterization of the peroxidase activityof CYP119, a thermostable P450 from Sulfolobus acidocaldarius (2008), ChemBioChem, 9, 420-425 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 9.2 | - |
Styrene | cosubstrate tert-butyl hydroperoxide, pH 8.5, 70°C | Sulfolobus acidocaldarius |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus acidocaldarius | - |
- |
- |
| Sulfolobus acidocaldarius DSM 639 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the initial activities of CYP119 in the presence of tert-butyl hydroperoxide and cumene hydroperoxide are two- and fivefold higher than that observed with H2O2. tert-Butyl hydroperoxide increases both turnover rate and the stability of the enzyme under the oxidizing reaction conditions | Sulfolobus acidocaldarius | ? | - |
- |
|
| additional information | the initial activities of CYP119 in the presence of tert-butyl hydroperoxide and cumene hydroperoxide are two- and fivefold higher than that observed with H2O2. tert-Butyl hydroperoxide increases both turnover rate and the stability of the enzyme under the oxidizing reaction conditions | Sulfolobus acidocaldarius DSM 639 | ? | - |
- |
|
| styrene + cumen hydroperoxide | - |
Sulfolobus acidocaldarius | styrene epoxide + ? | - |
? | |
| styrene + cumen hydroperoxide | - |
Sulfolobus acidocaldarius DSM 639 | styrene epoxide + ? | - |
? | |
| styrene + tert-butyl hydroperoxide | - |
Sulfolobus acidocaldarius | styrene epoxide + ? | - |
? | |
| styrene + tert-butyl hydroperoxide | - |
Sulfolobus acidocaldarius DSM 639 | styrene epoxide + ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Sulfolobus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
about 10% of the activy at 75°C | Sulfolobus acidocaldarius |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
5 min, no loss of activity | Sulfolobus acidocaldarius |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
Styrene | cosubstrate tert-butyl hydroperoxide, pH 8.5, 70°C | Sulfolobus acidocaldarius | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Sulfolobus acidocaldarius |
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