Application | Comment | Organism |
---|---|---|
synthesis | phenylacetone monooxygenase (PAMO) catalyzes oxidation of ketones with molecular oxygen and NADPH with the formation of esters. PAMO demonstrates high catalytic constants in the oxidation of benzylacetone and other structurally similar aromatic ketones and can be used in the synthesis of flavoring substances, since the ester formed by benzylacetone oxidation has a pronounced fruity smell | Thermobifida fusca |
Cloned (Comment) | Organism |
---|---|
gene pamO, recombinant expression of His-tagged enzyme in three different forms (C, N, and L) concerning the position of His-tag in Escherichia coli strain BL21(DE3), the His-tag locates at the N- and C-termini of the enzyme (PAMO N and PAMO C, respectively), PAMO L describes a construct with the His-tag distanced from the enzyme C-terminus with an additional 19-amino acid sequence, the enzyme forms do not show great differences in catalytic activity. No use of the arabinose promoter in the expression system | Thermobifida fusca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetone + NADPH + H+ + O2 | Thermobifida fusca | - |
benzyl acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | Q47PU3 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no posttranslational modification in the PAMO proteins expressed in Escherichia coli cells | Thermobifida fusca |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography to near-homogeneity | Thermobifida fusca |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 62000, SDS-PAGE | Thermobifida fusca |
Synonyms | Comment | Organism |
---|---|---|
PAMO | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Thermobifida fusca | |
NADPH | - |
Thermobifida fusca |
General Information | Comment | Organism |
---|---|---|
physiological function | phenylacetone monooxygenase (PAMO) catalyzes oxidation of ketones with molecular oxygen and NADPH with the formation of esters | Thermobifida fusca |