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Literature summary for 1.14.13.9 extracted from

  • Wilson, K.; Mole, D.; Binnie, M.; Homer, N.; Zheng, X.; Yard, B.; Iredale, J.; Auer, M.; Webster, S.
    Bacterial expression of human kynurenine 3-monooxygenase Solubility, activity, purification (2014), Protein Expr. Purif., 95, 96-103 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.9

Cloned(Commentary)

Cloned (Comment) Organism
expression of active FLAG(TM)-tagged enzyme in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0068
-
 NADPH pH 7, 37°C, full length 6His-tagged enzyme Homo sapiens
0.0087
-
 NADPH pH 7, 37°C, full length 12His-tagged enzyme, FLAG tag removed during affinity purification Homo sapiens
0.1486
-
 L-kynurenine pH 7, 37°C, full length 6His-tagged enzyme Homo sapiens
0.153
-
 L-kynurenine pH 7, 37°C, full length 12His-tagged enzyme, FLAG tag removed during affinity purification Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
outer mitochondrial membrane
-
 Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens O15229
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-kynurenine + NADPH + H+ + O2
-
 Homo sapiens 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?

General Information

General Information Comment Organism
drug target the enzyme is a therapeutic target in several disease states, including Huntington's disease Homo sapiens
metabolism the enzyme is central to the kynurenine pathway of tryptophan metabolism Homo sapiens