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Literature summary for 1.14.13.9 extracted from
- Functional characterization and mechanism of action of recombinant human kynurenine 3-hydroxylase (2000), Eur. J. Biochem., 267, 1092-1099.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| fusion protein with glutathione-S-transferase | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | 70% inhibition with 0.1 M NaCl or KCl, competitive with respect to NADPH and non-competitive with respect to L-kynurenine | Rattus norvegicus |  |
| pyridoxal | less potent than pyridoxal phosphate | Rattus norvegicus | |
| pyridoxal 5'-phosphate | non competitive inhibition | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrial outer membrane | - |
Rattus norvegicus | 5741 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Rattus norvegicus |
| 55760 | - |
calculated from primary sequence | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| fusion protein with gluthatione-S-transferase | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.028 | - |
NADPH oxidase activity | Rattus norvegicus |
| 2 | - |
fusion protein with glutathione-S-transferase | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C stable for 6 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-kynurenine + NADPH + O2 | - |
Rattus norvegicus | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
stable for four hours | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
fusion protein with glutathione-S-transferase | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | about 50% of maximal activity at pH 7 and 9 | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | one molecule non-covalently bound FAD per molecule of enzyme | Rattus norvegicus | |
| NADH | less effective than NADPH | Rattus norvegicus | |
| NADPH | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
| 1 | - |
pyridoxal | - |
Rattus norvegicus | |
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Release 2023.1 (January 2023)
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