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Literature summary for 1.14.13.9 extracted from
- The regulatory function of L-kynurenine 3-hydroxylase (EC 1.14.1.2) for the biosynthesis of pyridine nucleotides in anaerobically and aerobically grown Saccharomyces cerevisiae (1971), Hoppe-Seyler's Z. Physiol. Chem., 352, 1654-1658.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADH | above 3 mM | Saccharomyces cerevisiae |  |
| p-chloromercuribenzoate | weak, 50% inhibition at 0.4 M | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
NADPH | under aerobic conditions | Saccharomyces cerevisiae | |
| 0.017 | - |
NADPH | under unaerobic conditions | Saccharomyces cerevisiae | |
| 0.04 | - |
L-kynurenine | under aerobic conditions | Saccharomyces cerevisiae | |
| 0.045 | - |
L-kynurenine | under unaerobic conditions | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-kynurenine + NADPH + O2 | Saccharomyces cerevisiae | rate limiting step in the pyridine nucleotide biosynthesis from tryptophan | ? + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
mutant E105 has an enzymatic defect of kynurenine 3-hydroxylase | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme activity with different cofactors and under anaerobic conditions | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-kynurenine + NADPH + O2 | - |
Saccharomyces cerevisiae | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
| L-kynurenine + NADPH + O2 | rate limiting step in the pyridine nucleotide biosynthesis from tryptophan | Saccharomyces cerevisiae | ? + NADP+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.1 | 8.9 | about 50% of maximal activity at pH 7.1 and 8.9 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | less effective than NADPH | Saccharomyces cerevisiae | |
| NADPH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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