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Literature summary for 1.14.13.83 extracted from

  • Stamford, N.P.; Duggan, S.; Li, Y.; Alanine, A.I.; Crouzet, J.; Battersby, A.R.
    Biosynthesis of vitamin B12: the multi-enzyme synthesis of precorrin-4 and factor IV (1997), Chem. Biol., 4, 445-451.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene cobG heterologously expressed in Escherichia coli Pseudomonas denitrificans (nom. rej.)

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ iron-sulfur protein Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
precorrin-3A + NADH + H+ + O2 Pseudomonas denitrificans (nom. rej.) biosynthesis of vitamin B12 precorrin-3B + NAD+ + H2O aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 ir
precorrin-3A + NADH + H+ + O2 Pseudomonas denitrificans (nom. rej.) G3575 biosynthesis of vitamin B12 precorrin-3B + NAD+ + H2O aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 ir

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.)
-
-
-
Pseudomonas denitrificans (nom. rej.) G3575
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
precorrin-3A + NADH + H+ + O2
-
Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 ir
precorrin-3A + NADH + H+ + O2 biosynthesis of vitamin B12 Pseudomonas denitrificans (nom. rej.) precorrin-3B + NAD+ + H2O aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 ir
precorrin-3A + NADH + H+ + O2
-
Pseudomonas denitrificans (nom. rej.) G3575 precorrin-3B + NAD+ + H2O aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 ir
precorrin-3A + NADH + H+ + O2 biosynthesis of vitamin B12 Pseudomonas denitrificans (nom. rej.) G3575 precorrin-3B + NAD+ + H2O aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 ir

Synonyms

Synonyms Comment Organism
CobG
-
Pseudomonas denitrificans (nom. rej.)