Literature summary for 1.14.13.82 extracted from

Ravi, K.; Garcia-Hidalgo, J.; Brink, D.P.; Skyvell, M.; Gorwa-Grauslund, M.F.; Liden, G.
Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium (2019), Biores. Technol., 285, 121327 .

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Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
vanillate + O2 + NADH + H+	Microbacterium sp. RG1	-	3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde	-	?

Organism

Organism	UniProt	Comment	Textmining
Microbacterium sp. RG1	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	the cells grow in vanillate, 4-hydroxybenzoate, syringate, ferulate, p-coumarate, and 3-O-methylgallate, but not on vanillin and syringaldehyde. Maximum specific growth rates, uptake rates and yields on lignin model compounds by Microbacterium sp. RG1, overview. When strain is cultivated with 5 mM each of the aldehydes vanillin or syringaldehyde no growth is observed for more than 200 h	Microbacterium sp. RG1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
vanillate + O2 + NADH + H+	-	Microbacterium sp. RG1	3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NADH	-	Microbacterium sp. RG1

General Information

General Information	Comment	Organism
metabolism	softwood lignin is a polymer composed of mainly guaiacyl (G) units, and thus the most reported aromatic compounds generated from pretreatment of softwood-based lignin are guaiacyl-derivates such as vanillin and guaiacol. Hardwood lignin, on the other hand, contains both guaiacyl (G) and syringyl (S) units and its depolymerization results, apart from the guaiacyl-derivates, in S-derivates such as syringaldehyde, syringate and syringol. Yet, only a few organisms are reported to metabolize compounds from syringyl-based (S) lignin. Microbacterium sp. RG1 can grow on syringate and vanillate	Microbacterium sp. RG1

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Release 2023.1 (January 2023)