Cloned (Comment) | Organism |
---|---|
gene ysl1, DNA and amino acid sequence determination and analysis, genotyping, sequence comparisons and phylogenetic tree, complementation of the ysl1 mutant by complementation construct pC2300-YSL1 using the Agrobacterium tumefaciens transfection method, semiquantitative and quantitative real-time RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in rice protoplasts | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | yellow and spotted leaf 1 (ysl1) mutant in rice exhibiting a yellow-green leaf phenotype throughout the whole developmental stage and a spotted leaf phenotype at seedling and tillering stages. Genetic analysis indicates that a single recessive gene is responsible for the phenotype of ysl1 mutant. Map-based cloning shows that the mutation site is located at the region of 105.4-kb between RM572 and L3 on the short arm of chromosome 1. Sequence analysis reveals that a point mutation (C-to-T) happens in the coding region of candidate gene YSL1 (LOC_Os01g17170) which causes an amino acid change in MPEC. Mutant phenotype analysis. Growing under natural conditions, ysl1 mutant exhibits a yellow-green leaf in the whole developmental stage and slow growth comparing with its wild-type. Besides, the spots appear in the leaves of the mutant at seedling and tillering stages, are reduced at heading stage, and disappear at mature stage. Most of the main agronomic traits significantly decrease in the mutant. For example, plant height, number of productive panicles per plant, number of spikelets per panicle, seed setting rate, and 1000 grain weight decrease by 16.8%, 20.7%, 17.8%, 7.3%, and 10.6%, respectively, while the days to heading significantly increase by 12.1% | Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Oryza sativa Japonica Group | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Oryza sativa Japonica Group |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | Oryza sativa Japonica Group | overall reaction | 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q9FTU1 | cvs. Nipponbare or Minghui 63 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa Japonica Group | - |
additional information | YSL1 is expressed mainly in photosynthetic tissues and organs, almost no expression is detected in root, and its encoded protein is transported into the chloroplast with a signal peptide | Oryza sativa Japonica Group | - |
seedling | - |
Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | overall reaction | Oryza sativa Japonica Group | 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Mg-protoporphyrin IX monomethyl ester cyclase | - |
Oryza sativa Japonica Group |
MPEC | - |
Oryza sativa Japonica Group |
YSL1 | - |
Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa Japonica Group |
General Information | Comment | Organism |
---|---|---|
malfunction | the yellow and spotted leaf 1 (ysl1) mutant in rice exhibits a yellow-green leaf phenotype throughout the whole developmental stage and a spotted leaf phenotype at seedling and tillering stages. The phenotype of ysl1 is caused by a single nucleotide substitution of Mg-protoporphyrin IX monomethyl ester cyclase YSL1 which is allelic to YGL8, OsCRD1, and YL-1 | Oryza sativa Japonica Group |
metabolism | Mg-protoporphyrin IX monomethyl ester cyclase (MPEC) plays an essential role in chlorophyll biosynthesis | Oryza sativa Japonica Group |
physiological function | Mg-protoporphyrin IX monomethyl ester cyclase (MPEC) plays an essential role in chlorophyll biosynthesis | Oryza sativa Japonica Group |