Literature summary for 1.14.13.81 extracted from

Kong, W.; Yu, X.; Chen, H.; Liu, L.; Xiao, Y.; Wang, Y.; Wang, C.; Lin, Y.; Yu, Y.; Wang, C.; Jiang, L.; Zhai, H.; Zhao, Z.; Wan, J.
The catalytic subunit of magnesium-protoporphyrin IX monomethyl ester cyclase forms a chloroplast complex to regulate chlorophyll biosynthesis in rice (2016), Plant Mol. Biol., 92, 177-191 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene ygl8 encodes a catalytic subunit of MgPME cyclase, DNA and amino acid sequence determination and analysis, map-based cloning, sequence comparisons and phylogenetic analysis. Transient expression in Nicotiana benthamiana. The recombinant construct pC1305-YGL8 is introduced into the ygl8 rice mutant by Agrobacterium tumefaciens EHA105-mediated transformation. Quantitative real-time PCR expression analysis	Oryza sativa

Cloned (Comment)

Organism

gene ygl8 encodes a catalytic subunit of MgPME cyclase, DNA and amino acid sequence determination and analysis, map-based cloning, sequence comparisons and phylogenetic analysis. Transient expression in Nicotiana benthamiana. The recombinant construct pC1305-YGL8 is introduced into the ygl8 rice mutant by Agrobacterium tumefaciens EHA105-mediated transformation. Quantitative real-time PCR expression analysis

Oryza sativa

Protein Variants

Protein Variants	Comment	Organism
additional information	isolation of an Oryza sativa yellow-green leaf 8 (ygl8) mutant that exhibits chlorosis phenotype with abnormal chloroplast development in young leaves. During the development of leaves, the chlorotic plants turn green accompanied by restorations in chlorophyll content and chloroplast ultrastructure. Expression levels of some nuclear genes associated with Chl biosynthesis are affected in both the ygl8 mutant and YGL8 RNA interference lines. Phenotype, overview. Ygl8 knockout by RNAi	Oryza sativa
N182S	the ygl8 mutation causes a conserved amino acid substitution, which is related to the alterations of chlorophyll precursor content	Oryza sativa

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast membrane	the YGL8 subunit contains a chloroplast transit peptide	Oryza sativa	31969	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2	Oryza sativa	-	3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Oryza sativa	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	young, high enzyme content	Oryza sativa	-
additional information	subunit YGL8 is constitutively expressed in various tissues, with more abundance in young leaves and panicles	Oryza sativa	-
panicle	high enzyme content	Oryza sativa	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2	-	Oryza sativa	3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O	-	?

Synonyms

Synonyms	Comment	Organism
magnesium-protoporphyrin IX monomethyl ester cyclase	-	Oryza sativa
MgPME cyclase	-	Oryza sativa

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Oryza sativa

General Information

General Information	Comment	Organism
malfunction	Oryza sativa yellow-green leaf 8 (ygl8) mutant exhibits a chlorosis phenotype with abnormal chloroplast development in young leaves. During the development of leaves, the chlorotic plants turn green accompanied by restorations in chlorophyll content and chloroplast ultrastructure, expression levels of some nuclear genes associated with Chl biosynthesis are affected in both the ygl8 mutant and YGL8 RNA interference lines. YGL8 expression demonstrates no differences between the wild-type and ygl8 mutant plants, but the mutant shows increase of Proto IX and MgP/MgPME expression, accompanied with the decrease of protochlorophyllide. Phenotype, overview	Oryza sativa
additional information	protein YGL8 has the dual functions in chlorophyll biosynthesis: one as a catalytic subunit of MgPME cyclase, the other as a core component of FLU-YGL8-LCAA-POR complex in chlorophyll biosynthesis, physical interaction between YGL8 and a rice chloroplast protein, low chlorophyll accumulation A (OsLCAA). YGL8 also interacts with the other two rice chloroplast proteins, viz. fluorescent (OsFLU1) and NADPH:protochlorophyllide oxidoreductase (OsPORB)	Oryza sativa