Literature summary for 1.14.13.81 extracted from

Yang, L.; Lou, Y.; Peng, Z.; Zhao, H.; Sun, H.; Gao, Z.
Molecular characterization and primary functional analysis of PeMPEC, a magnesium-protoporphyrin IX monomethyl ester cyclase gene of bamboo (Phyllostachys edulis) (2015), Plant Cell Rep., 34, 2001-2011 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene PeMPEC, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant overexpression in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV310 transformation method. Gene PeMPEC driven by the CAMV 35S promoter is transferred into the Crd1 mutant leading to functional complementation of the mutant, the chlorophyll concentration of sense plants is 22-50% higher than that of the Crd1 mutant, and 7-20% higher than that of wild-type Col-0	Phyllostachys edulis

Cloned (Comment)

Organism

gene PeMPEC, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant overexpression in Arabidopsis thaliana Col-0 using the Agrobacterium tumefaciens strain GV310 transformation method. Gene PeMPEC driven by the CAMV 35S promoter is transferred into the Crd1 mutant leading to functional complementation of the mutant, the chlorophyll concentration of sense plants is 22-50% higher than that of the Crd1 mutant, and 7-20% higher than that of wild-type Col-0

Phyllostachys edulis

Protein Variants

Protein Variants	Comment	Organism
additional information	chlorophyll fluorescence analysis of Arabidopsis wild-type Col-0 and PeMPEC transgenic plants, overview	Phyllostachys edulis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	the enzyme sequence contains a transit peptide	Phyllostachys edulis	-	-

Organism

Organism	UniProt	Comment	Textmining
Phyllostachys edulis	A0A0K1L5Y4	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	transcription of PeMPEC occurs mainly in the leaf	Phyllostachys edulis	-
additional information	very low or no enzyme expression in root, stem, and sheath	Phyllostachys edulis	-
seedling	-	Phyllostachys edulis	-

Subunits

Subunits	Comment	Organism
?	x * 47900, about, proenzyme, sequence calculation, x * 42700, about, mature enzyme, sequence calculation, x * 62000, recombinant tagged enzyme, SDS-PAGE	Phyllostachys edulis

Synonyms

Synonyms	Comment	Organism
magnesium-protoporphyrin IX monomethyl ester cyclase	-	Phyllostachys edulis
MPEC	-	Phyllostachys edulis
PeMPEC	-	Phyllostachys edulis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Phyllostachys edulis	sequence calculation	-	9.1

Expression

Organism	Comment	Expression
Phyllostachys edulis	temperature has a temporally sensitive and complex effect on PeMPEC transcription	additional information

General Information

General Information	Comment	Organism
physiological function	MPEC is an essential enzyme in chlorophyll biosynthesis	Phyllostachys edulis