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Literature summary for 1.14.13.81 extracted from

  • Bollivar, D.; Braumann, I.; Berendt, K.; Gough, S.P.; Hansson, M.
    The Ycf54 protein is part of the membrane component of Mg-protoporphyrin IX monomethyl ester cyclase from barley (Hordeum vulgare L.) (2014), FEBS J., 281, 2377-2386 .
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
XanL required by the enzyme, found associated with the membrane and protein Ycf54 Hordeum vulgare
Ycf54 identified based upon strong association of Ycf54 with XanL. Active Ycf54 is membrane localized. The conserved chloroplast polypeptide Ycf54 is involved in the MPEC-enzyme catalyzed reaction. Sequencing, recombinant expression as His-tagged protein, and purification, UniProt ID F2D891 Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast membrane
-
 Hordeum vulgare 31969
-

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 cv. Bonus
-

Synonyms

Synonyms Comment Organism
Mg-protoporphyrin IX monomethyl ester cyclase
-
 Hordeum vulgare
MPEC
-
 Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Hordeum vulgare

General Information

General Information Comment Organism
malfunction Hordeum vulgare mutants viridis-k, light green 3, light green 4 and zebra stripe 2 are not deficient in Ycf54. Both xanthan-l and viridis-k membranes are unable to support MPEC activity when combined with wild-type soluble fraction Hordeum vulgare
physiological function enzyme MPEC requires components found in both the membrane and soluble fractions of the chloroplast. It requires components associated with the plastid membrane and the plastid soluble fraction for activity. One of the components, XanL is found associated with the membrane and another protein, Ycf54 has been identified based upon its strong association of with XanL. The conserved chloroplast polypeptide Ycf54 is involved in the MPEC-enzyme catalyzed reaction Hordeum vulgare