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Literature summary for 1.14.13.81 extracted from

  • Wong, Y.S.; Castelfranco, P.A.
    Resolution and reconstitution of magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, the enzyme system responsible for the formation of the chlorophyll isocyclic ring (1984), Plant Physiol., 75, 658-661.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.81

Activating Compound

Activating Compound Comment Organism Structure
ATP requirement for reconstituted system Cucumis sativus
S-adenosyl-L-methionine 1 mM, absolute requirement when magnesium-protoporphyrin IX is used as substrate Cucumis sativus

Inhibitors

Inhibitors Comment Organism Structure
methyl viologen at micromolar concentrations Cucumis sativus
methylene blue at micromolar concentrations Cucumis sativus
phenazine methosulfate at micromolar concentrations Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Cucumis sativus 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 Cucumis sativus
-
 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of the enzyme system from a high-speed supernatant and a membrane pellet Cucumis sativus

Storage Stability

Storage Stability Organism
-15°C, 30% loss of activity in supernatant, 70% loss of activity in pellet after 7 days Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
magnesium-protoporphyrin IX + NADPH + O2
-
 Cucumis sativus ? + NADP+ + H2O
-
 ?
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2
-
 Cucumis sativus 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O
-
 ?
additional information overview: tetrapyrrole with substituents at various positions Cucumis sativus ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Cucumis sativus
NADPH more effective than NADH below 2 mM, equally effective at 10 mM Cucumis sativus