Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.8 extracted from

  • Inoue, S.; Morita, R.; Minami, Y.
    An indigo-producing plant, Polygonum tinctorium, possesses a flavin-containing monooxygenase capable of oxidizing indole (2021), Biochem. Biophys. Res. Commun., 534, 199-205 .
    View publication on PubMed
Search for more literature for 1.14.13.8

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of enzyme PtFMO in Escherichia coli in presence of tryptophan leading to formation of indigo, coexpression of PtFMO with indoxyl beta-D-glucoside synthase (PtIGS), which catalyzes the glucosylation of indoxyl, brings about the formation of indican Persicaria tinctoria

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
indole + NADPH + H+ + O2 Persicaria tinctoria
-
 indole N-oxide + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Persicaria tinctoria
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf in three-week-old Polygonum tinctorium specimens, the first leaves demonstrate higher levels of PtFMO expression than the subsequent leaves Persicaria tinctoria
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
indole + NADPH + H+ + O2
-
 Persicaria tinctoria indole N-oxide + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
flavin-containing monooxygenase
-
 Persicaria tinctoria
PtFMO
-
 Persicaria tinctoria

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Persicaria tinctoria
NADPH
-
 Persicaria tinctoria

Expression

Organism Comment Expression
Persicaria tinctoria tryptophan induces the enzyme up