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Literature summary for 1.14.13.8 extracted from

  • Krueger, S.K.; Henderson, M.C.; Siddens, L.K.; VanDyke, J.E.; Benninghoff, A.D.; Karplus, P.A.; Furnes, B.; Schlenk, D.; Williams, D.E.
    Characterization of sulfoxygenation and structural implications of human flavin-containing monooxygenase isoform 2 (FMO2.1) variants S195L and N413K (2009), Drug Metab. Dispos., 37, 1785-1791.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.8

Activating Compound

Activating Compound Comment Organism Structure
cholate activates activity of mutant N413K by 23% Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, and genotyping Homo sapiens

Protein Variants

Protein Variants Comment Organism
N413K naturally occuring mutant of the FMO2*1 allele, the mutant shows higher kcat and Vmax, and increased thermosensitivity compared to the wild-type enzyme, activity is stabilized by NADPH Homo sapiens
Q472X naturally occuring mutant of the FMO2*1 allele, more frequent in the sub-Sahara African population Homo sapiens
S195L naturally occuring mutant of the FMO2*1 allele, the mutant shows reduced activity and increased pH sensitivity and thermosensitivity compared to the wild-type enzyme, activity is stabilized by NADPH Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0048
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant N413K Homo sapiens
0.005
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant S195L Homo sapiens
0.0068
-
 NADPH pH 9.0, 37°C, mutant N413K Homo sapiens
0.0115
-
 NADPH pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
0.0135
-
 methyl p-tolyl sulfide pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
0.132
-
 NADPH pH 9.0, 37°C, mutant S195L Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 activates activity of mutant N413K by 18% Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q99518
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl p-tolyl sulfide + NADPH + H+ + O2
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More structure comparison of wild-type and mutant enzymes, crystal structure analysis, overview Homo sapiens

Synonyms

Synonyms Comment Organism
FMO2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 loss of about 30% of activity after heat treatment of mutant enzyme N413K, activity is preservable by NADPH Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant S195L Homo sapiens
15.2
-
 NADPH pH 9.0, 37°C, mutant S195L Homo sapiens
23.6
-
 NADPH pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
42.3
-
 methyl p-tolyl sulfide pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
71.1
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant N413K Homo sapiens
94.4
-
 NADPH pH 9.0, 37°C, mutant N413K Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 higher activity compared to pH 8.5 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9.5
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information FMO2 contains no bound FAD Homo sapiens
NADPH
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00014
-
 NADPH pH 9.0, 37°C, mutant S195L Homo sapiens
0.0022
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant S195L Homo sapiens
0.0033
-
 methyl p-tolyl sulfide pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
0.0068
-
 NADPH pH 9.0, 37°C, wild-type FMO2.1 Homo sapiens
0.0144
-
 NADPH pH 9.0, 37°C, mutant N413K Homo sapiens
0.0149
-
 methyl p-tolyl sulfide pH 9.0, 37°C, mutant N413K Homo sapiens