BRENDA - Enzyme Database show
show all sequences of 1.14.13.79

The pea gene NA encodes ent-kaurenoic acid oxidase

Davidson, S.E.; Elliott, R.C.; Helliwell, C.A.; Poole, A.T.; Reid, J.B.; Plant Physiol. 131, 335-344 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
both genes PsKAO1 and PsKAO2 encode the ent-kaurenoic acid oxidase, expression in Saccharomyces cerevisiae
Pisum sativum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
Pisum sativum
-
gibberellin A12 aldehyde + NADP+ + 2 H2O
-
-
?
ent-kaur-16-en-19-oate + NADPH + H+ + O2
Pisum sativum
-
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
-
-
?
gibberellin A12 aldehyde + NADPH + O2
Pisum sativum
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pisum sativum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
apical bud
expression of the gene PsKAO1
Pisum sativum
-
leaf
expression of the gene PsKAO1
Pisum sativum
-
pod
expression of the gene PsKAO1
Pisum sativum
-
root
expression of the gene PsKAO1
Pisum sativum
-
seed
developing, expression of the gene PsKAO1, the gene PsKAO1 is expressed only in seed
Pisum sativum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
-
440386
Pisum sativum
gibberellin A12 aldehyde + NADP+ + 2 H2O
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440386
Pisum sativum
gibberellin A12 aldehyde + NADP+ + 2 H2O
-
-
-
?
ent-kaur-16-en-19-oate + NADPH + H+ + O2
-
440386
Pisum sativum
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-en-19-oate + NADPH + H+ + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440386
Pisum sativum
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
gibberellin A12 aldehyde + NADPH + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440386
Pisum sativum
gibberellin A12 + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P450 protein
Pisum sativum
Cloned(Commentary) (protein specific)
Commentary
Organism
both genes PsKAO1 and PsKAO2 encode the ent-kaurenoic acid oxidase, expression in Saccharomyces cerevisiae
Pisum sativum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P450 protein
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
Pisum sativum
-
gibberellin A12 aldehyde + NADP+ + 2 H2O
-
-
?
ent-kaur-16-en-19-oate + NADPH + H+ + O2
Pisum sativum
-
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
-
-
?
gibberellin A12 aldehyde + NADPH + O2
Pisum sativum
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
apical bud
expression of the gene PsKAO1
Pisum sativum
-
leaf
expression of the gene PsKAO1
Pisum sativum
-
pod
expression of the gene PsKAO1
Pisum sativum
-
root
expression of the gene PsKAO1
Pisum sativum
-
seed
developing, expression of the gene PsKAO1, the gene PsKAO1 is expressed only in seed
Pisum sativum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
-
440386
Pisum sativum
gibberellin A12 aldehyde + NADP+ + 2 H2O
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + NADPH + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440386
Pisum sativum
gibberellin A12 aldehyde + NADP+ + 2 H2O
-
-
-
?
ent-kaur-16-en-19-oate + NADPH + H+ + O2
-
440386
Pisum sativum
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
ent-kaur-16-en-19-oate + NADPH + H+ + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440386
Pisum sativum
ent-7alpha-hydroxykaur-16-en-19-oate + NADP+ + H2O
-
-
-
?
gibberellin A12 aldehyde + NADPH + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440386
Pisum sativum
gibberellin A12 + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.13.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
727594
Yang
Molecular evolution of the ent ...
Chikusichloa aquatica, Ehrharta erecta, Leersia tisserantii, Luziola fluitans, Oryza brachyantha, Oryza meridionalis, Oryza meyeriana var. granulata, Oryza officinalis, Oryza punctata, Oryza sativa, Rhynchoryza subulata
Genet. Mol. Biol.
35
448-454
2012
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13
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700131
Khlestkina
-
Ent-kaurenoic acid oxidase gen ...
Aegilops tauschii, Triticum aestivum, Triticum urartu
Mol. Breed.
25
251-258
2009
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3
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11
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11
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11
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11
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11
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11
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3
11
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688097
Sawada
Germination of photoblastic le ...
Lactuca sativa
J. Exp. Bot.
59
3383-3393
2008
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1
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1
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3
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2
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1
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1
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1
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2
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1
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660237
Sakamoto
An overview of gibberellin met ...
Oryza sativa
Plant Physiol.
134
1642-1653
2004
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1
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3
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3
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4
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3
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1
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1
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1
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3
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4
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3
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440386
Davidson
The pea gene NA encodes ent-ka ...
Pisum sativum
Plant Physiol.
131
335-344
2003
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1
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3
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3
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5
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5
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1
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1
1
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3
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5
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5
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440385
Helliwell
The CYP88A cytochrome P450, en ...
Arabidopsis thaliana, Hordeum vulgare, Zea mays
Proc. Natl. Acad. Sci. USA
98
2065-2070
2001
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2
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9
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8
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9
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3
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2
3
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9
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9
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