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Literature summary for 1.14.13.7 extracted from
- Phenol hydroxylase (1962), Biochim. Biophys. Acta, 62, 423-426.
General Stability
| General Stability | Organism |
|---|---|
| dilution causes considerable loss of activity and cannot be prevented by addition of proteins such as egg or serum albumin or substances of high molecular weight such as Carbowax-4000 or polyvinylpyrrolidone | Brevibacterium fuscum |
| partially purified enzyme loses considerable activity upon dialysis or aging, addition of boiled extract prepared from crude extract fully restores activity | Brevibacterium fuscum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| copper-chelating agents | - |
Brevibacterium fuscum | |
| glutathione | - |
Brevibacterium fuscum |  |
| potassium ethylxanthate | 1 mM | Brevibacterium fuscum | |
| Sodium diethyldithiocarbamate | 1 mM | Brevibacterium fuscum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | participation of Cu2+ in reaction | Brevibacterium fuscum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacterium fuscum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Brevibacterium fuscum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-amino-3-methylphenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| 2-aminophenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| 2-chlorophenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| 2-methyl-phenol + O2 + NADPH | i.e. o-cresol | Brevibacterium fuscum | ? | - |
? | |
| 3-aminophenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| 3-chlorophenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| 3-methylphenol + O2 + NADPH | i.e. m-cresol | Brevibacterium fuscum | ? | - |
? | |
| 4-aminophenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| 4-chlorophenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| 4-methyl-phenol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| additional information | not: p-hydroxyphenylacetic acid | Brevibacterium fuscum | ? | - |
? | |
| additional information | not: 2,4-, 2,5- and 2,6-dimethylphenols | Brevibacterium fuscum | ? | - |
? | |
| additional information | not: p-hydroxybenzoic acid | Brevibacterium fuscum | ? | - |
? | |
| additional information | not: salicylic acid | Brevibacterium fuscum | ? | - |
? | |
| orcinol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| phenol + NADPH + O2 | - |
Brevibacterium fuscum | catechol + NADP+ + H2O | - |
? | |
| phloroglucinol + O2 + NADPH | - |
Brevibacterium fuscum | ? | - |
? | |
| resorcinol + NADPH + O2 | - |
Brevibacterium fuscum | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Brevibacterium fuscum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | less active than NADPH | Brevibacterium fuscum | |
| NADPH | - |
Brevibacterium fuscum | |
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Release 2023.1 (January 2023)
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