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Literature summary for 1.14.13.61 extracted from

  • Rosche, B.; Tshisuaka, B.; Fetzner, S.; Lingens, F.
    2-Oxo-1,2-dihydroquinoline 8-monooxygenase,a two-component enzyme system from Pseudomonas putida 86 (1995), J. Biol. Chem., 270, 17836-17842.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Polyethylene glycol enhances activity Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Pseudomonas putida
4,5-dihydroxy-1,3-benzene disulfonic acid
-
Pseudomonas putida
EDTA
-
Pseudomonas putida
iodoacetate
-
Pseudomonas putida
NEM
-
Pseudomonas putida
p-hydroxymercuribenzoate
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ enhances activity Pseudomonas putida
Iron the reductase component contains 1 plant-type ferredoxin [2Fe-2S] cluster, the oxygenase component contains 6 Rieske-type [2Fe-2S]clusters and additional iron Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE Pseudomonas putida
39000
-
reductase component, gel filtration Pseudomonas putida
55000
-
the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE Pseudomonas putida
330000
-
oxygenase component, gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-Oxo-1,2-dihydroquinoline + NADH + O2 Pseudomonas putida second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline ?
-
?
2-Oxo-1,2-dihydroquinoline + NADH + O2 Pseudomonas putida 86 second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida 86
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.97
-
oxygenase component Pseudomonas putida
32.5
-
reductase component Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Oxo-1,2-dihydroquinoline + NADH + O2 second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline Pseudomonas putida ?
-
?
2-Oxo-1,2-dihydroquinoline + NADH + O2 second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline Pseudomonas putida 86 ?
-
?
2-Oxo-1,2-dihydroquinoline + NADH + O2 no catalytic activity under anaerobic conditions Pseudomonas putida 8-Hydroxy-2-oxo-1,2-dihydroquinoline + NAD+ + H2O
-
?
2-Oxo-1,2-dihydroquinoline + NADH + O2 no catalytic activity under anaerobic conditions Pseudomonas putida 86 8-Hydroxy-2-oxo-1,2-dihydroquinoline + NAD+ + H2O
-
?
additional information the reductase component shows NADH:acceptor reductase activity with: cytochrome c, ferricyanide, 2,6-dichlorophenol indophenol and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida ?
-
?
additional information the reductase component shows NADH:acceptor reductase activity with: cytochrome c, ferricyanide, 2,6-dichlorophenol indophenol and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride Pseudomonas putida 86 ?
-
?

Subunits

Subunits Comment Organism
? the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD the reductase component contains 1 FAD Pseudomonas putida