Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Polyethylene glycol | enhances activity | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Pseudomonas putida | |
4,5-dihydroxy-1,3-benzene disulfonic acid | - |
Pseudomonas putida | |
EDTA | - |
Pseudomonas putida | |
iodoacetate | - |
Pseudomonas putida | |
NEM | - |
Pseudomonas putida | |
p-hydroxymercuribenzoate | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | enhances activity | Pseudomonas putida | |
Iron | the reductase component contains 1 plant-type ferredoxin [2Fe-2S] cluster, the oxygenase component contains 6 Rieske-type [2Fe-2S]clusters and additional iron | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE | Pseudomonas putida |
39000 | - |
reductase component, gel filtration | Pseudomonas putida |
55000 | - |
the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE | Pseudomonas putida |
330000 | - |
oxygenase component, gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Oxo-1,2-dihydroquinoline + NADH + O2 | Pseudomonas putida | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | ? | - |
? | |
2-Oxo-1,2-dihydroquinoline + NADH + O2 | Pseudomonas putida 86 | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 86 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.97 | - |
oxygenase component | Pseudomonas putida |
32.5 | - |
reductase component | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-Oxo-1,2-dihydroquinoline + NADH + O2 | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | Pseudomonas putida | ? | - |
? | |
2-Oxo-1,2-dihydroquinoline + NADH + O2 | second enzyme in the quinoline degradation pathway. The reductase component and the oxygenase component are inducible by the substrate 2-oxo-1,2-dihydroquinoline | Pseudomonas putida 86 | ? | - |
? | |
2-Oxo-1,2-dihydroquinoline + NADH + O2 | no catalytic activity under anaerobic conditions | Pseudomonas putida | 8-Hydroxy-2-oxo-1,2-dihydroquinoline + NAD+ + H2O | - |
? | |
2-Oxo-1,2-dihydroquinoline + NADH + O2 | no catalytic activity under anaerobic conditions | Pseudomonas putida 86 | 8-Hydroxy-2-oxo-1,2-dihydroquinoline + NAD+ + H2O | - |
? | |
additional information | the reductase component shows NADH:acceptor reductase activity with: cytochrome c, ferricyanide, 2,6-dichlorophenol indophenol and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida | ? | - |
? | |
additional information | the reductase component shows NADH:acceptor reductase activity with: cytochrome c, ferricyanide, 2,6-dichlorophenol indophenol and 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride | Pseudomonas putida 86 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | the reductase component is a monomer, 1 * 37000, SDS-PAGE. The oxygenase component is a hexamer, 6 * 55000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the reductase component contains 1 FAD | Pseudomonas putida |